HigherPurity™ Blood/Cultured Cell Total RNA Kit

Name: HigherPurity™ Blood/Cultured Cell Total RNA Kit
SKU: AN0145
Availability: InStock

For a Highly Efficient, Easy & Convenient Total RNA from fresh whole Human Blood and Cultured cells

The kit is a highly efficient, easy and convenient HigherPurity™ Blood/Cultured Cell Total RNA Kit designed specifically for purifying Total RNA from fresh whole Human blood and cultured cells.

It is based in the use of special detergents and chaotropic salt to lyse cells and inactivate RNase. Once all contaminants have been removed the purified Total RNA is eluted by RNase-Free Water.

Price: 165.53 € – 669.78 €

size

50 rxn

100 rxn

250 rxn

SKU: AN0145 Categories: DNA & RNA Extraction, RNA Spin Column based Purification

Detailed information:

Advantages & Features

Highly efficient: yields up to 2 μg from whole blood or up to 30 μg from 1×10⁶ 293T cells.
Easy and fast protocol: ready-to-use Total RNA in 19 minutes.
Convenient: flexible column applicability (centrifugation and vaccum).
Optimized spin colums: for Total RNA extraction.
High Binding capacity: < 100 μg Total RNA/column.
Minimal Sample Volume: less of 300 μl whole blood is needed.
Minimum elution volume: 40 μL.

Specifications

Includes

Includes for 50 rxn:
– 120 mL RL Buffer
– 25 mL Buffer
– 30 mL Wash Buffer 1
– 15 mL Wash Buffer 2 (concentrate)
– 10 mL RNase-free Water
– 50 RNAprep spin column
– 50 Filter Column
– 100 Collection Tube
– 50 Elution Tube

Download documentation

Datasheet

MSDS

Applications

RT-PCR.
Northern Blotting.
Primer Extension.
mRNA Selection.
cDNA Synthesis.

Tables & Figures

Quality Control

Total RNA is isolated from 300 μl of fresh whole Human blood.
Purified RNA is quantified using a spectrophotometer with a typical yield of more than 2 μg of Total RNA and an A260/A280 ratio of 1.8-2.0.
Quality is further checked by agarose gel electrophoresis.

Advice

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage: All components can be stored at Room Temperature (15 – 25 °C).

Citations

Κορμέντζα, Ελισάβετ. “Μεταβολή της βιολογικής συμπεριφοράς των καρκινικών κυττάρων 22Rv1, πριν και μετά την εγκατάσταση αλλομοσχεύματος σε ανοσοανεπαρκή θήλεα και άρρενα ποντίκια SCID.“ (2021).
Παπαδάκη, Ε. (2021). Σύγκριση της βιολογικής συμπεριφοράς των καρκινικών ορμονοευαίσθητων κυττάρων προστάτη 22Rv1, πριν και μετά την εγκατάσταση αλλομοσχεύματος σε ανοσοανεπαρκείς μύες.
Μωυσέως, Ά. (2021). Σύγκριση της βιολογικής συμπεριφοράς των καρκινικών ορμονοάντοχων κυττάρων προστάτη DU145, πρίν και μετά την εγκατάσταση αλλομοσχεύματος σε SCID-mice.
Giatromanolaki, A., Fasoulaki, V., Kalamida, D., Mitrakas, A., Kakouratos, C., Lialiaris, T., & Koukourakis, M. I. (2019). CYP17A1 and Androgen-Receptor Expression in Prostate Carcinoma Tissues and Cancer Cell Lines. Current Urology, 13(3), 157-165.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.