qMAXSen™ One-Step Green RT-qPCR Kit (High Rox™)
For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube
qMAXSen™ One-Step Green RT-qPCR Kit (High Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 4x concentration to perform Real-Time PCR using a proprietary green dye. The qPCR MasterMix contains all the reagent (except PCR Primers and Template) needed for running PCR reactions. In addition, a separate RT mix that comprises a balanced mixture of both RTase and RNase Inhibitor is also supplied.
Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.
Price: 157.00 € – 534.94 €
Detailed information:
Advantages & Features
- Enhanced specificity, sensitivity, and yields in cDNA Synthesis.
- Versatile: compatible with most used real-time PCR instruments.
- Convenient: includes internal passive reference dye.
- Easy: reaction assembly.
Specifications
Includes
Includes for 500 rxn (of 20 µl):
– 5 x 500 µL qMAXSen™ One-Step RT-qPCR (4x)
– 5 x 100 μL RT mix
– 5 x 1 mL RNase-free Water
Download documentation
Datasheet MSDSApplications
- Detection and Quantification of DNA and cDNA targets.
- Gene Expression.
- Standard and fast qPCR platforms.
- High Throughput Applications.
Tables & Figures
Quality Control
- Functionally tested in RT qPCR based on specific probe for activity, processivity, efficiency, sensitivity and heat.
Advice
Storage, Shipping & Guarantee
- Shipment at Gel Pack.
- Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.
Citations
Ruiz, Leticia, et al. “Resistance to Cucumber Green Mottle Mosaic Virus in Cucumis melo.” Plants 10.6 (2021): 1077.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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