Poly A Polymerase
High Quality & Purity Enzyme manufactured from Recombinant source
Poly(A) Polymerase I from Escherichia coli, is a very active recombinant enzyme produced by a overproducer strain of E. coli. The enzyme adds a long tail of adenine nucleotides at the 3’ end of messenger RNA using ATP as donor.
GMP-grade is also available. Learn more.
Detailed information:
Advantages & Features
- High-Purity: ≥ 95% determined by SDS.
- Recombinant: isolated from E. coli.
- Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
- GMP-grade available.
Specifications
Protein information
Predicted MW: 54 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
Buffer Storage: 1X Reaction Buffer: 20 mM Tris-HCl, 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1 % Triton®X-100, 50% Glycerol, (pH 7.5 at 25°C).
Biological activity: One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37°C.
Gene Information
Synonyms: PAP I
Official Symbol: pcnB
Specie: Escherichia coli
GenBank accession number: NC_000913 (Range 157729..159126)
NCBI Protein RefSeq: NP_414685.4
Protein Family: polyA polymerase
Includes
– 100 U Poly(A) Polymerase, from E. coli (5 U/µL)
–Â Adenosine-5′-Triphosphate (ATP)
–Â Poly(A) Polymerase Reaction Buffer
Download documentation
Datasheet MSDSApplications
- Labeling of RNA with ATP or cordycepin.
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhances translation of RNA transferred into eukaryotic cells.
Tables & Figures
Quality Control
Advice
Avoid exposure to constant temperature changes.
Storage, Shipping & Guarantee
- Shipped in:Â Gel Pack.
- Storage:Â -20 ÂşC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
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