HotBegan™ Hot Start Taq-DNA Polymerase
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg
HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Price: 71.05 €
Detailed information:
Advantages & Features
- High specificity: minimizes unspecific amplification.
- Efficient: prevents or minimizes primer-dimer and nonspecific products.
- Great sensitivity: amplifies from femptograms of DNA targets.
- Inactive: at Room Temperature.
- Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
- Powerful: amplification of targets up to 5 kb.
- Convenient: available in kits and MasterMix solution.
Specifications
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Includes
– 100 μL HotBegan™ Hot Start Taq DNA Polymerase (5 U/μL)
– 25 mM MgCl2 (1.5 mL)
– 1.5 mL Buffer (10x)
Download documentation
Datasheet MSDSApplications
- qPCR.
- PCR and RT-PCR.
- Genotyping with Taqman Probes.
- PCR fragments amplification for TA or GC cloning.
- Amplification from a limited DNA template or low copy number genes.
Tables & Figures
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Quality Control
- Functionally tested in qPCR.
- None detected bacterial DNA (by qPCR).
- Exempt of nucleases (endo-, exo and ribonucleases) activity guaranteed by appropriate quality tests.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- Mora-Sala, B., León, M., Pérez-Sierra, A., & Abad-Campos, P. (2022). New Reports of Phytophthora Species in Plant Nurseries in Spain. Pathogens, 11(8), 826.
- Aigoun-Mouhous, Wassila, et al. “Cadophora sabaouae sp. nov. and Phaeoacremonium species associated with Petri disease on grapevine propagation material and young grapevines in Algeria.” Plant Disease ja (2021).
- León, Maela, et al. “Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain.“ Agronomy 10.8 (2020): 1062.
- Albuquerque, C. A. D. (2019). Detection and characterization of Dichelobacter nodosus from sheep with different clinical manifestations of Ovine Footrot (Doctoral dissertation).
- Roque, J. M. L. (2019). Mecanismos virucidas da irradiação por feixe de eletrões em adenovírus humanos (Doctoral dissertation).
- Elena, G., Berbegal, M., González-Domínguez, E., & Armengol, J. (2019). Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain. European Journal of Plant Pathology, 1-12.
- Osset Iborra, H. (2019). Seguimiento de las plagas de Tomicus destruens y Orthotomicus erosus y detección de Botryosphaeriaceae, hongos patógenos asociados a estos insectos, en los pinares de Pinus halepensis de La Pedrera y la Devesa del Saler (Valencia) (Doctoral dissertation).
- Olmo, D., Armengol, J., León, M., & Gramaje, D. (2016). Characterization and pathogenicity of Botryosphaeriaceae species isolated from almond trees on the island of Mallorca (Spain). Plant disease, 100(12), 2483-2491.
- Negreiros, A. M., Júnior, R. S., Rodrigues, A. P., León, M., & Armengol, J. Prevalent weeds collected from cucurbit fields in Northeastern Brazil reveal new species diversity in the genus Monosporascus. Annals of Applied Biology.
- Martín-Pinto, P. Fungal communities from forest systems in Ethiopia.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession under Eucalyptus grandis plantations in Ethiopia. Forest Ecology and Management, 405, 179-187.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.
- Huyghe, J. (2017). Selection of reference genes for gene expression studies in Lactic acid bacteria.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal community succession and sporocarp production following fire occurrence in Dry Afromontane forests of Ethiopia. Forest Ecology and Management, 398, 37-47.
- Criado-García, J., Fuentes, F., Cruz-Teno, C., García-Rios, A., Jiménez-Morales, A., Delgado-Lista, J., … & Pérez-Jiménez, F. (2011). R353Q polymorphism in the factor VII gene and cardiovascular risk in Heterozygous Familial Hypercholesterolemia: a case-control study. Lipids in health and disease, 10(1), 50.
- Català, S., Pérez-Sierra, A., & Abad-Campos, P. (2015). The use of genus-specific amplicon pyrosequencing to assess Phytophthora species diversity using eDNA from soil and water in northern Spain. PloS one, 10(3), e0119311.
- Olmo, D., Armengol, J., León, M., & Gramaje, D. (2016). Characterization and Pathogenicity of Botryosphaeriaceae Species Isolated from Almond Trees on the Island of Mallorca (Spain). Plant Disease, 100(12), 2483-2491.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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