CVX™ T7 ARCA mRNA Synthesis Kit
For Easy, Scalable & High-Quality in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase
CVX™ T7 ARCA mRNA Synthesis Kit is designed for in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA). ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated.
By using a DNA template encoding a poly(A) tail, the CVX™ T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs.
mRNAs synthesized with the CVX™ T7 ARCA mRNA Kit can subsequently be used for cell transfection, microinjection, in vitro translation and RNA vaccines. The kit contains sufficient reagents for 50 reactions of 20 μl each. A 20 μl reaction yields about 50 μg RNA with 1 μg of template added (1.5 kb RNA transcript). The T7 RNA Polymerase Enzyme mix includes Ribonuclease Inhibitor Protein to protect newly transcribed RNA from degradation by RNases.
Detailed information:
Advantages & Features
- High-Quality Kit: High enzymatic purity, specificity, and activity.
- Scaleable: Increase the reaction size to produce milligram amounts of RNA.
- Easy and time-saving protocol with minimal handling steps.
- Convenient: Rnase Inhibitor included.
Specifications
Includes
– 3 x 40 μl T7 RNA Polymerase mix (include RNase Inhibitor)
– 200 μl T7 Reaction Buffer 10x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 10 µl ARCA Solution (100mM)
– 10 µl T7 Control template 2 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
- Synthesis of ARCA-capped (m)RNA.
- Aantisense RNA and RNAi experiments.
- RNA vaccines.
- RNA structure and function studies.
Tables & Figures
Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
- Avoid freeze/thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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