CANFAST™ Transfection Reagent

Name: CANFAST™ Transfection Reagent
SKU: T0083
Price: 50.43 EUR
Availability: InStock

For a Highly Efficient, Easy & Non-toxic High-throughput Transfection

CANFAST™ is a Highly Efficient, Ready-to-& Non-Toxic new generation cationic Polymer. It has important features as DNA condensation and endosomal release, which improves gene transfection efficiency.

1 mL

Price: 50.43 €

SKU: T0083 Category: Cell Transfection

Detailed information:

Advantages & Features

High transfection efficiency and reproducibility in most common cell lines, both adherent and suspension cell lines.
Non-toxic: minimal cytotoxicity with a cell survival rate higher than 90%.
Serum-compatible in cell cultures.
Easy and time-saving protocol: just 24 minutes for successful transfections with minimal handling.
Optimized: direct application for most cell lines.
Proven performance: for transient and stable transfections, adherent and suspension cells.
Cost effective: minimal amount of DNA required and allows use of the same medium after transfection.

Specifications

Includes

-1 mL CANFAST™ Transfection Reagent (1 µg/µL)
-10 µL GFP-Plasmid Transfection Control (1 µg/µL)

(1 mL = 250 – 500 transfections)

Download documentation

Datasheet

MSDS

Brochure

Applications

High-throughput transfection.
Stable and transient transfections.
Co-transfection.
Transfection of primary cells and cell lines.
Transfection of adherent and suspension cells.
All cellular analysis applications.

Tables & Figures

Quality Control

Transfection range of Green fluorescent Protein Expression vector in JURKAT cell line is >50%.

Advice

Storage, Shipping & Guarantee

Shipped in: Ambient temperature.
Storage: 4 ºC.

Citations

Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Caballero-Villarraso, J., … & Paz-Rojas, E. (2022). Development of a High-Throughput Calcium Mobilization Assay for CCR6 Receptor Coupled to Hydrolase Activity Readout. Biomedicines, 10(2), 422.
Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Túnez-Fiñana, I., & Paz-Rojas, E. (2021). Amino Terminal Recognition by a CCR6 Chemokine Receptor Antibody Blocks CCL20 Signaling and IL-17 Expression via β-arrestin.
Yago-Ibáñez, J., García-Pastor, C., Lucio-Cazaña, F. J., & Fernández-Martínez, A. B. (2020). Retinoic acid receptor-beta prevents cisplatin-induced proximal tubular cell death. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 165795.
Pilná, H. (2019). Vliv interferon regulujícího faktoru 3 na imunitní odpověď proti viru vakcínie v atopickém organismu.
Olmos, G., Martínez‐Miguel, P., Alcalde‐Estevez, E., Medrano, D., Sosa, P., Rodríguez‐Mañas, L., … & López‐Ongil, S. (2017). Hyperphosphatemia induces senescence in human endothelial cells by increasing endothelin‐1 production. Aging Cell.
Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.