FDG – Galactosidase Assay Kit
Optimized, Stable & Convenient tool to quickly measure the levels of active β-galactosidase
FDG β-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of fluorescein di-β-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of β-galactosidase is proportional to fluorescence produced.measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Price: 290.50 €
Detailed information:
Advantages & Features
- Fast and convenient.
- Easy-to-use method to quantify the enzyme expression in transfected cells.
- Sensitive: measure ß-galactosidase at femtogram level.
Specifications
Includes
– 55 mL Reaction Buffer
– 25 mL Lysis Buffer
– 25 mL Stop solution
– 20 μL β-galactosidase enzyme (0.1 mg/mL)
– 500 μL β-Mercaptoethanol
– 125 μL FDG solution (10 mM)
Download documentation
Datasheet MSDSApplications
- Measurement of β-Galactosidase activity in the lysates of transfected cell.
Tables & Figures
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Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: Upon receipt, store the kit at -20 ºC. β-galactosidase substrate must be protected from light.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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