Horse-Power™ Green-Taq DNA Polymerase
Horse-Power Taq DNA Polymerase is a thermostable recombinant enzyme produced in an E. coli strain, that carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
The 10X Green Buffer contains an agarose loading buffer including two tracking dyes (blue and yellow dye) for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well agarose gels. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel).
Price: 57.30 €
Detailed information:
Advantages & Features
• Molecular Weight: 94 kDa.
• Thermostable (half life at 94ºC is 40 minute).
• Adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´ overhangs PCR fragments. This fact allows the popular TA-cloning or GC cloning.
• Incorporates modified nucleotides (biotinylated, fluorescently labeled, etc)
• Save time – go directly from PCR to gel electrophoresis.
Specifications
Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mg MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Includes
• 100 µL Horse-Power Taq DNA polymerase (5U/µL).
• 2 x 1.25 mL 10X Green Buffer
Download documentation
Datasheet MSDSApplications
• Routine amplifications.
• Colony screening.
• Amplifications up to 6 kb using plasmid, viral, or genomic DNA as a template.
• PCR fragments amplification for TA or GC cloning
Tables & Figures
Quality Control
• Functionally tested in PCR.
• Undetected bacterial DNA (by PCR).
• Undetectable nucleases activity (endo-, exo- and ribonucleases).
Advice
Storage, Shipping & Guarantee
• Shipped in: Gel pack.
• Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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