Annexin V-APC Apoptosis Detection Kit
For a Convenient, Easy & Fast method for Apoptosis Detection by Flow Cytometry and Fluorescence Microscopy
Annexin V-APC Apoptosis Detection Kit are a convenient, easy-to-use and safe method for Apoptosis Detection. Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS).
Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry.
Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.
Price: 218.23 €
Detailed information:
Advantages & Features
- Easy and one step protocol: get results with few handling steps.
- Detection Method: Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy.
- Mammalian Reactivity.
- Proven performance for both adherent and suspension cells.
- Versatile: most used kits availables.
- Safe: non-enzymatic assay that avoids fixation.
Specifications
Includes
-500 µL Labeled Annexin V-APC
-50 mL Binding Buffer (10x)
-1 mL Propidium Iodide
Download documentation
Datasheet MSDSApplications
- Detect early/middle stages of Apoptosis.
- Differentiate apoptosis from necrosis.
Tables & Figures
Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: All components should be stored at 4 ºC.
- Recommendations: Protect from light and do not freeze.
Citations
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- Punginelli, D., Catania, V., Abruscato, G., Luparello, C., Vazzana, M., Mauro, M., … & Schillaci, D. (2023). New Bioactive Peptides from the Mediterranean Seagrass Posidonia oceanica (L.) Delile and Their Impact on Antimicrobial Activity and Apoptosis of Human Cancer Cells. International Journal of Molecular Sciences, 24(6), 5650.
- Carrión-Marchante, R., Pinto-Díez, C., Klett-Mingo, J. I., Palacios, E., Barragán-Usero, M., Pérez-Morgado, M. I., … & Martín, M. E. (2023). An Aptamer against MNK1 for Non-Small Cell Lung Cancer Treatment. Pharmaceutics, 15(4), 1273.
- Vašícek, J., Baláži, A., Svoradová, A., Vozaf, J., Dujícková, L., Makarevich, A. V., … & Chrenek, P. Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers. Int. J. Mol. Sci.
- Tirpák, F., Halo, M., Tokárová, K., Binkowski, L. J., Vašíček, J., Svoradová, A., … & Massányi, P. (2021). Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality. Life, 11(11), 1238.
- Křen, V. Antioxidative effect of dietary flavonoid isoquercitrin on human ovarian granulosa.
- Luparello, C., Ragona, D., Asaro, D. M. L., Lazzara, V., Affranchi, F., Arizza, V., & Vazzana, M. (2020). Cell-Free Coelomic Fluid Extracts of the Sea Urchin Arbacia lixula Impair Mitochondrial Potential and Cell Cycle Distribution and Stimulate Reactive Oxygen Species Production and Autophagic Activity in Triple-Negative MDA-MB231 Breast Cancer Cells. Journal of Marine Science and Engineering, 8(4), 261.
- José, M., Grueso, L., María, R., Valero, T., Beatriz, C. H., Daniel, L. R., … & Alicia, P. C. (2019). Peroxiredoxin 6 Down-Regulation Induces Metabolic Remodeling and Cell Cycle Arrest in HepG2 Cells. Antioxidants, 8(11), 505.
- Luparello, C., Ragona, D., Asaro, D. M. L., Lazzara, V., Affranchi, F., Celi, M., … & Vazzana, M. (2019). Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells. Biology, 8(4), 76.
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Vašíček, J., Baláži, A., & Chrenek, P. (2019). STAUROSPORINE-INDUCED APOPTOSIS ANALYSED BY LOW-COST AND HIGH-COST ANNEXIN V ASSAYS. Slovak Journal of Animal Science, 52(02), 47-52.
- García-Pastor, C., Blázquez-Serra, R., Bosch, R. J., Cazaña, F. J. L., & Fernández-Martínez, A. B. (2019). Apoptosis and cell proliferation in proximal tubular cells exposed to apoptotic bodies. Novel pathophysiological implications in cisplatin-induced renal injury. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease.
- Fernández‐Bedmar, Z., Anter, J., & Alonso Moraga, A. (2018). Anti/genotoxic, longevity inductive, cytotoxic, and clastogenic‐related bioactivities of tomato and lycopene. Environmental and molecular mutagenesis, 59(5), 427-437.
- Luparello, C., Asaro, D. M. L., Cruciata, I., Hassell-Hart, S., Sansook, S., Spencer, J., & Caradonna, F. (2019). Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells. International Journal of Molecular Sciences, 20(4), 804.
- Fernández‐Bedmar, Z., Anter, J., & Moraga, Á. A. (2018). Anti/genotoxic, longevity inductive, cytotoxic, and clastogenic‐related bioactivities of tomato andlycopene. Environmental and molecular mutagenesis.
- Martínez-Valdivieso, D., Font, R., Fernández-Bedmar, Z., Merinas-Amo, T., Gómez, P., Alonso-Moraga, Á., & Del Río-Celestino, M. (2017). Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Antigenotoxicity, Cytotoxicity and Apoptotic Effects. Nutrients, 9(7), 755.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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