XTT Cell Proliferation Assay Kit

Name: XTT Cell Proliferation Assay Kit
SKU: CA031
Availability: InStock

For an Optimized, Accurate & Sensitive Colorimetric Detection of Cellular Metabolic Activities

XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells.

This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample. The formazan dye formed in the assay is soluble in aqueous solution and quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulphate), can significantly improve the efficiency of XTT reduction in cells.

Price: 99.89 € – 189.65 €

size

500 assays

1000 assays

SKU: CA031 Categories: Cell Based Assays, Proliferation Assays

Detailed information:

Advantages & Features

Accurate: dye absorbance is proportional to the number of cells in each well.
Sensitive: assayed even in low cell concentrations.
Fast protocol: results within 2-5 hours with minimal handling steps.
Time-saving protocol: avoids solubilisation step.
Complete solution: includes all reagents needed for cell washing procedures.
Safe: avoids radioactivity.
Optimized: for high throughput assays (no washing or other steps that can cause cell loss and variability).
Cost avoidance: allows performance directly in a microtiter plate.

Specifications

Includes

Includes for 1,000 assays:
– 2 x 25 mL XTT Cell Proliferation Assay Kit Reagent
– 1 mL Activation Reagent

Download documentation

Datasheet

MSDS

Applications

Spectrophotometric quantification of cell proliferation and viability in response to pharmaceutical, chemical, nutrients and environmental compounds.
High Throughput Screening.

Tables & Figures

Quality Control

Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Advice

Storage, Shipping & Guarantee

Shipped at: Gel Pack.
Storage: -20 °C.
Recommendations: Protect XTT solution from light.

Citations

Tavares Pereira, M., Kazemian, A., Rehrauer, H., & Kowalewski, M. P. (2022). Transcriptomic profiling of canine decidualization and effects of antigestagens on decidualized dog uterine stromal cells. Scientific Reports, 12(1), 21890.
Kuczwara, V., Schuler, G., Pfarrer, C., Tiedje, L., Kazemian, A., Pereira, M. T., … & Klisch, K. (2023). Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition. Veterinary Sciences, 10(1), 44.
Hervás-Corpión, I., Navarro-Calvo, J., Martín-Climent, P., Iriarte-Gahete, M., Geribaldi-Doldán, N., Castro, C., & Valor, L. M. (2023). Defining a Correlative Transcriptional Signature Associated with Bulk Histone H3 Acetylation Levels in Adult Glioblastomas. Cells, 12(3), 374.
Zaballos, M. A., Acuña-Ruiz, A., Morante, M., Riesco-Eizaguirre, G., Crespo, P., & Santisteban, P. (2022). Inhibiting ERK dimerization ameliorates BRAF-driven anaplastic thyroid cancer. Cellular and Molecular Life Sciences, 79(9), 1-19.
Ramírez-Moya, J., Wert-Lamas, L., Acuña-Ruíz, A., Fletcher, A., Wert-Carvajal, C., McCabe, C. J., … & Riesco-Eizaguirre, G. (2022). Identification of an interactome network between lncRNAs and miRNAs in thyroid cancer reveals SPTY2D1-AS1 as a new tumor suppressor. Scientific reports, 12(1), 1-13.
Ramírez-Moya, Julia, et al. “An ADAR1-dependent RNA editing event in the cyclin-dependent kinase CDK13 promotes thyroid cancer hallmarks.” Molecular Cancer 20.1 (2021): 1-20.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.