SEAP Reporter Gene Assay Kit

Name: SEAP Reporter Gene Assay Kit
SKU: CA040
Price: 333.25 EUR
Availability: InStock

For a really Easy, Sensitive & Fast Assay, Luminescence based, Measurement the levels of SEAP in the Culture medium

Secreted Alkaline Phosphatase (SEAP) Reporter Gene is a really easy, sensitive and fast assay that utilizes enzyme activity of alkaline phosphatase to dephosphorylate the chemiluminescent alkaline phosphatase substrate CSPD into an unstable dioxetane anion which decomposes and emits light.

SEAP encodes a truncated form of the placental enzyme that lacks the membrane anchoring domain, thereby allowing the protein to secrete efficiently from transfected cells.

Changes in levels of SEAP activity detected in the culture medium are directly proportional to changes in intracellular concentrations of SEAP mRNA and protein.

288 assays

Price: 333.25 €

SKU: CA040 Categories: Cell Based Assays, Reporter Gene Assays

Detailed information:

Advantages & Features

Convenient: a single set of cells is used for both the SEAP assay and another purpose.
Time-saving protocol: results in 55 minutes due the elimination of cell lysate preparation.
Cost-effective: allows performance directly in a microtiter plate.
Sensitive: assayed even in low cell concentrations.
Secreted from transfected cells into the culture medium.

Specifications

Includes

– 3 x 96 W Solid Plate (white)
– 3 units of lid
– 50 μL Alkaline Phosphatase Standard
– 15 mL SEAP Substrate (Luminescence)

Download documentation

Datasheet

MSDS

Applications

Measurement the levels of SEAP in the culture medium of transfected cells.

Tables & Figures

Quality Control

Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Advice

Precautions: Some components of this kit are potentially carcinogenic or corrosive; it is advisable to work in a hood and to wear glasses, gloves and a mask.

Storage, Shipping & Guarantee

Shipping: Ambient Temperature.
Storage: 4 ºC, except Component solution containing CSPD® Substrate (2-6 °C), Placental Alkaline Phosphatase Positive Control (-20 °C).
Recommendations: Protect SEAP Substrate from light.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.