pSpark® I
For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use
pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency.
The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.
Price: 175.00 €
Detailed information:
Advantages & Features
- Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
- Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
- Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
- High stability: eliminates cloning bias or pitfalls.
- Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert.
- Compatible with blue/white screening.
- Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
- Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
- Eliminates positive selection vector.
- High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
- Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
Specifications
Includes
– 20 rxn pSpark® I (20 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)
Applications
- General cloning.
- Cloning of High Fidelity PCR amplified products.
- Production of ssDNA.
- Blue/white screening for recombinants.
- In vitro transcription from T7/SP6 dual-opposed promoters.
Tables & Figures
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Quality Control
- Functional test using a 1.0 kb PCR fragment.
Advice
- Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above -20 °C inactivates the enzyme.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
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- Moll, L., Giralt, N., Planas, M. et al. Prunus dulcis response to novel defense elicitor peptides and control of Xylella fastidiosa infections. Plant Cell Rep 43, 190 (2024).
- Delgado García, E. (2023). Caracterización de la familia génica de las nucleosidasas en Phaseolus vulgaris.
- Paullada‐Salmerón, J. A., Wang, B., & Muñoz‐Cueto, J. A. (2023). Spexin in the European sea bass, Dicentrarchus labrax: Characterization, brain distribution, and interaction with Gnrh and Gnih neurons. Journal of Comparative Neurology, 531(2), 314-335.
- Huertas-García, A. B., Guzmán, C., Tabbita, F., & Alvarez, J. B. (2022). Allelic variation of puroindolines genes in Iranian common wheat landraces. Agriculture, 12(8), 1196.
- Gómez Gil, L. (2022). Structure and dynamics of chromosomes: role in the genomic and pathogenic plasticity of Fusarium oxysporum.
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- Morales de los Ríos Martín, L. (2022). Influencia del transportador de nitrato NRT1. 4/NPF6. 2 en la nutrición de potasio en Arabidopsis thaliana.
- Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
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- Ruiz Beltrán, C., Nadal i Matamala, A., Montesinos Seguí, E., & Pla i de Solà-Morales, M. (2018). Novel Rosaceae Plant Elicitor Peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp. © Molecular Plant Pathology, 2018, vol. 19, núm. 2, p. 418-431.
- Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Identification and Characterization of TALE Homeobox Genes in the Endangered Fern Vandenboschia speciosa. Genes, 8(10), 275.
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- Aliaga‐Guerrero, M., Paullada‐Salmerón, J. A., Piquer, V., Mañanós, E. L., & Muñoz‐Cueto, J. A. (2018). Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects. Journal of Comparative Neurology, 526(2), 349-370.
- Muñoz-Jiménez, C., Ayuso, C., Dobrzynska, A., Torres-Mendéz, A., de la Cruz Ruiz, P., & Askjaer, P. (2017). An efficient FLP-based toolkit for spatiotemporal control of gene expression in Caenorhabditis elegans. Genetics, 206(4), 1763-1778.
- Madroñal de Sancha, J. M. (2017). Estudios funcionales de las pirofosfatasas inorgánicas de saccharomyces cerevisiae y organismos fotosintéticos.
- Álvarez Cao, M. E. (2017). Optimización de la producción heteróloga de la enzima α-galactosidasa de” Saccharomyces cerevisiae” a partir de residuos agroindustriales.
- Atanasov, K. E., Liu, C., Erban, A., Kopka, J., Parker, J. E., & Alcázar, R. (2018). NLR mutations suppressing immune hybrid incompatibility and their effects on disease resistance. Plant Physiology, pp-00462.
- Reñé, A., Alacid, E., Figueroa, R. I., Rodríguez, F., & Garcés, E. (2017). Life-cycle, ultrastructure, and phylogeny of Parvilucifera corolla sp. nov.(Alveolata, Perkinsozoa), a parasitoid of dinoflagellates. European journal of protistology, 58, 9-25.
- Burnat, M., Li, B., Kim, S. H., Michael, A. J., & Flores, E. Homospermidine biosynthesis in the cyanobacterium Anabaena requires a deoxyhypusine synthase homologue and is essential for normal diazotrophic growth. Molecular Microbiology.
- Rengel, R., Smith, R. T., Haslam, R. P., Sayanova, O., Vila, M., & León, R. (2018). Overexpression of acetyl-CoA synthetase (ACS) enhances the biosynthesis of neutral lipids and starch in the green microalga Chlamydomonas reinhardtii. Algal Research, 31, 183-193.
- Domínguez-Martín, M. A., López-Lozano, A., Clavería-Gimeno, R., Velázquez-Campoy, A., Seidel, G., Burkovski, A., … & García-Fernández, J. M. (2017). Differential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus. Frontiers in Microbiology, 8, 2641.
- Calatrava, V., Hom, E. F., Llamas, Á., Fernández, E., & Galván, A. (2018). Ok, thanks! A new mutualism between Chlamydomonas and Methylobacteria facilitates growth on amino acids and peptides. FEMS Microbiology Letters.
- Velázquez-Palmero, D., Romero-Segura, C., García-Rodríguez, R., Hernández, M. L., Vaistij, F. E., Graham, I. A., … & Martínez-Rivas, J. M. (2017). An Oleuropein β-Glucosidase from Olive Fruit Is Involved in Determining the Phenolic Composition of Virgin Olive Oil. Frontiers in plant science, 8.
- Bornikoel, J., Carrión, A., Fan, Q., Flores, E., Forchhammer, K., Mariscal, V., … & Maldener, I. (2017). Role of Two Cell Wall Amidases in Septal Junction and Nanopore Formation in the Multicellular Cyanobacterium Anabaena sp. PCC 7120. Frontiers in cellular and infection microbiology, 7, 386.
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- Badosa, E., Montesinos, L., Camó, C., Ruz, L., Cabrefiga, J., Francés, J., … & Feliu, L. (2017). CONTROL OF FIRE BLIGHT INFECTIONS WITH SYNTHETIC PEPTIDES THAT ELICIT PLANT DEFENSE RESPONSES. Journal of Plant Pathology, 99, 65-73.
- De Felipe, A. P., Lamas, J., Sueiro, R. A., Folgueira, I., & Leiro, J. M. (2017). New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species. Parasitology, 144(10), 1394-1411.
- Suarez‐Bregua, P., Chien, C. J., Megias, M., Du, S., & Rotllant, J. (2017). Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish. Developmental Dynamics, 246(12), 992-1000.
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- Martínez-Barriocanal, Á., Arcas-García, A., Magallon-Lorenz, M., Ejarque-Ortíz, A., Negro-Demontel, M. L., Comas-Casellas, E., … & Martín, M. (2017). Effect of Specific Mutations in Cd300 Complexes Formation; Potential Implication of Cd300f in Multiple Sclerosis. Scientific Reports, 7(1), 13544.
- Bornikoel, J., Carrión, A., Fan, Q., Flores, E., Forchhammer, K., Mariscal, V., … & Maldener, I. (2017). Role of Two Cell Wall Amidases in Septal Junction and Nanopore Formation in the Multicellular Cyanobacterium Anabaena sp. PCC 7120. Frontiers in cellular and infection microbiology, 7.
- Badosa, E., Montesinos, L., Camó, C., Ruz, L., Cabrefiga, J., Francés, J., … & Feliu, L. (2017). CONTROL OF FIRE BLIGHT INFECTIONS WITH SYNTHETIC PEPTIDES THAT ELICIT PLANT DEFENSE RESPONSES. Journal of Plant Pathology, 99.
- Suarez‐Bregua, P., Chien, C. J., Megias, M., Du, S. D., & Rotllant, J. (2017). Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish. Developmental Dynamics.
- Munoz-Jimenez, C. M., Ayuso, C., Dobrzynska, A., Torres, A., de la Cruz-Ruiz, P., & Askjaer, P. (2017). An efficient FLP-based toolkit for spatiotemporal control of gene expression in Caenorhabditis elegans. bioRxiv, 107029.
- Pilar Prieto-Dapena, Concepción Almoguera, José-María Personat, Francisco Merchan, Juan Jordano; Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis, Journal of Experimental Botany, Volume 68, Issue 5, 1 February 2017, Pages 1097–1108, https://doi.org/10.1093/jxb/erx020
- Ruiz-Roldán, C., Pareja-Jaime, Y., González-Reyes, J. A., & G. Roncero, M. I. (2015). The transcription factor Con7-1 is a master regulator of morphogenesis and virulence in Fusarium oxysporum. Molecular Plant-Microbe Interactions, 28(1), 55-68.
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- Burnat, M., Schleiff, E., & Flores, E. (2014). Cell envelope components influencing filament length in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of bacteriology, 196(23), 4026-4035.
- Rodríguez, M. F. R., Sánchez-García, A., Salas, J. J., Garcés, R., & Martínez-Force, E. (2015). Characterization of soluble acyl-ACP desaturases from Camelina sativa, Macadamia tetraphylla and Dolichandra unguis-cati. Journal of plant physiology, 178, 35-42.
- Rodríguez-Rodríguez, M. F., Salas, J. J., Venegas-Calerón, M., Garcés, R., & Martínez-Force, E. (2016). Molecular cloning and characterization of the genes encoding a microsomal oleate Δ 12 desaturase (CsFAD2) and linoleate Δ 15 desaturase (CsFAD3) from Camelina sativa. Industrial Crops and Products, 89, 405-415.
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- Chamizo-Ampudia, A., Galvan, A., Fernandez, E., & Llamas, A. (2011). The Chlamydomonas reinhardtii molybdenum cofactor enzyme crARC has a Zn-dependent activity and protein partners similar to those of its human homologue. Eukaryotic cell, 10(10), 1270-1282.
- Merino-Puerto, V., Herrero, A., & Flores, E. (2013). Cluster of genes that encode positive and negative elements influencing filament length in a heterocyst-forming cyanobacterium. Journal of bacteriology, 195(17), 3957-3966.
- Merino-Puerto, V., Herrero, A., & Flores, E. (2013). Cluster of genes that encode positive and negative elements influencing filament length in a heterocyst-forming cyanobacterium. Journal of bacteriology, 195(17), 3957-3966.
- Rodríguez-Rodríguez, M. F., Salas, J. J., Garcés, R., & Martínez-Force, E. (2014). Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition. Phytochemistry, 107, 7-15.
- Olaya-Abril, A., Obando, I., & Rodríguez-Ortega, M. J. (2016). Data in support of proteomic analysis of pneumococcal pediatric clinical isolates to construct a protein array. Data in brief, 6, 917-922.
- Olaya-Abril, A., Jiménez-Munguía, I., Gómez-Gascón, L., Obando, I., & Rodríguez-Ortega, M. J. (2015). A pneumococcal protein array as a platform to discover serodiagnostic antigens against infection. Molecular & Cellular Proteomics, 14(10), 2591-2608.
- Olaya-Abril, A., Jiménez-Munguía, I., Gómez-Gascón, L., Obando, I., & Rodríguez-Ortega, M. J. (2013). Identification of potential new protein vaccine candidates through pan-surfomic analysis of pneumococcal clinical isolates from adults. PloS one, 8(7), e70365.
- Jiménez-Munguía, I., van Wamel, W. J., Olaya-Abril, A., García-Cabrera, E., Rodríguez-Ortega, M. J., & Obando, I. (2015). Proteomics-driven design of a multiplex bead-based platform to assess natural IgG antibodies to pneumococcal protein antigens in children. Journal of Proteomics, 126, 228-233.
- Burnat, M., & Flores, E. (2014). Inactivation of agmatinase expressed in vegetative cells alters arginine catabolism and prevents diazotrophic growth in the heterocyst‐forming cyanobacterium Anabaena. MicrobiologyOpen, 3(5), 777-792.
- Ejarque-Ortiz, A., Solà, C., Martínez-Barriocanal, Á., Schwartz Jr, S., Martín, M., Peluffo, H., & Sayós, J. (2015). The receptor CMRF35-like molecule-1 (CLM-1) enhances the production of LPS-induced pro-inflammatory mediators during microglial activation. PloS one, 10(4), e0123928.
- Munoz-Jimenez, C. M., Ayuso, C., Dobrzynska, A., Torres, A., de la Cruz-Ruiz, P., & Askjaer, P. (2017). An efficient FLP-based toolkit for spatiotemporal control of gene expression in Caenorhabditis elegans. bioRxiv, 107029.
- Gómez-Marín, C., Tena, J. J., Acemel, R. D., López-Mayorga, M., Naranjo, S., de la Calle-Mustienes, E., … & Bovolenta, P. (2015). Evolutionary comparison reveals that diverging CTCF sites are signatures of ancestral topological associating domains borders. Proceedings of the National Academy of Sciences, 112(24), 7542-7547.
- Rojas, M. (2014). Papel de los reguladores moleculares Fbp1 y Bmh2 en la virulencia de Fusarium oxysporum.
- VIDAL, A. G. (2016). Potencial de los tratamientos por termoterapia con agua caliente para el control de hongos de la madera de la vid y su efecto sobre la micoflora total, después de un año de cultivo.
- Corral Ramos, C. (2016). Metabolismo del glucógeno y procesos celulares implicados en dinámica nuclear y fusión de hifas en Fusarium oxysporum.
- Navarro Velasco, G. Y. (2013). Identificación de nuevos componentes de la ruta TOR de Fusarium oxysporum y determinación de su papel en la patogénesis.
- Ruiz-Roldán, C., Pareja-Jaime, Y., González-Reyes, J. A., & G. Roncero, M. I. (2015). The transcription factor Con7-1 is a master regulator of morphogenesis and virulence in Fusarium oxysporum. Molecular Plant-Microbe Interactions, 28(1), 55-68.
- Jiménez Munguía, I. (2015). Multi-omic approach applied to the selection of vaccine antigens and molecules for diagnosis and treatment agianst caused by Streptococcus pneumoniae and Staphylococcus aureus.
- Nürnberg, D. J. (2015). Intercellular communication in filamentous cyanobacteria
- Lambert Rodríguez, R. (2016). Actividad nucleasa en judía y su relación con la síntesis de ureidos durante la germinación y senescencia.
- Ocaña Calahorro, F. J. (2013). El óxido nítrico y la asmilación de nitrógeno en Chlamydomonas.
- Domínguez Martín, M. A. (2015). Diversity of regulatory mechanisms in the C/N metabolism of the marine cyanobacteria Prochlorococcus and synechococcus.
- Sein-Echaluce, V. C., Castejón, M. F. F., & Rodríguez, A. G. (2012). Estudios funcionales de la proteína FurB en Anabaena sp. PCC 7120.
- Bravo Ruiz, G. A. (2013). Sistemas hidrolíticos de componentes vegetales en el patógeno de tomate Fusarium oxysporum f. sp. lycopersici: lipasas y poligalaturonasas.
- Chamizo-Ampudia, A., Galvan, A., Fernandez, E., & Llamas, A. (2011). The Chlamydomonas reinhardtii molybdenum cofactor enzyme crARC has a Zn-dependent activity and protein partners similar to those of its human homologue. Eukaryotic cell, 10(10), 1270-1282.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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