pSpark® Universal

– 20 rxn pSpark® II DNA cloning vector (20ng/µL)
– 20 rxn pSpark® TA DNA cloning vector (50ng/µL)
– 150 µL PEG 6000 solution (10X)
– 5 µL control insert 1kb
– 5 µLcontrol insert (TA) 600kb
– 20 µL T4 DNA ligase (5 Weiss U/µL)
– 100 µLT4 DNA ligase Buffer (10x)

• Cloning of high fidelity PCR amplified products into pSpark® II Blunt DNA cloning vector.
• Cloning of non-proofreading PCR fragments into pSpark® TA DNA Cloning vector.
• Production of ssDNA.
• In vitro transcription from T7/SP6 dual-opposed promoters.

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• Functional test using a 1.0 kb PCR fragment.
• Functional test using a 600 pb PCR fragment.

• Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

• Shipped in: Gel Pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

• Alvarez, J. B., Castellano, L., Recio, R., & Cabrera, A. (2019). Wx Gene in Hordeum chilense: Chromosomal Location and Characterisation of the Allelic Variation in the Two Main Ecotypes of the Species. Agronomy, 9(5), 261.
• Benítez Casanova, L. (2017). Caracterización de las principales enzimas celulolíticas de myceliophthora thermophila implicadas en la degradación de biomasa lignocelulósica y mejora de la hidrólisis de hemicelulosa para la producción de bioetanol de segunda generación.
• Oliveras Rovira, À., Baró, A., Montesinos Barreda, L., Feliu Soley, L., & Planas i Grabuleda, M. (2018). Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens. PLoS ONE, 2018, vol. 13, núm. 7, p. e0201571.
• Folgueira, I., Lamas, J., De Felipe, A. P., Sueiro, R. A., & Leiro, J. M. (2019). Evidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite. BioRxiv, 607622.
• Olmos, G., Martínez‐Miguel, P., Alcalde‐Estevez, E., Medrano, D., Sosa, P., Rodríguez‐Mañas, L., … & López‐Ongil, S. (2017). Hyperphosphatemia induces senescence in human endothelial cells by increasing endothelin‐1 production. Aging cell, 16(6), 1300-1312.
• Laguna, J. I. R. (2017). Proliferative and Positional Instructions Underliying Planarian Regeneration and Tissue Renewal(Doctoral dissertation, Universitat de Barcelona).
• Lindo, L., McCormick, S. P., Cardoza, R. E., Kim, H. S., Brown, D. W., Alexander, N. J., … & Gutierrez, S. (2019). Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux. Fungal Genetics and Biology, 122, 31-46.
• Oliveras, À., Baró, A., Montesinos, L., Badosa, E., Montesinos, E., Feliu, L., & Planas, M. (2018). Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens. PloS one, 13(7), e0201571.
• Álvarez-Cao, M. E., Cerdán, M. E., González-Siso, M. I., & Becerra, M. (2019). Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol. Frontiers in microbiology, 10.
• Álvarez-Escribano, I., Vioque, A., & Muro-Pastor, A. M. (2018). NsrR1, a nitrogen stress-repressed sRNA, contributes to the regulation of nblA in Nostoc sp. PCC 7120. Frontiers in microbiology, 9, 2267.
• TC, G. Supplemental Material. González-‐Aguilera C. et al.
• Aliaga‐Guerrero, M., Paullada‐Salmerón, J. A., Piquer, V., Mañanós, E. L., & Muñoz‐Cueto, J. A. (2017). Gonadotropin‐inhibitory hormone in the flatfish, Solea Senegalensis: Molecular cloning, brain localization and physiological effects. Journal of Comparative Neurology.
• Reñé, A., Alacid, E., Figueroa, R. I., Rodríguez, F., & Garcés, E. (2016). Life-cycle, ultrastructure, and phylogeny of Parvilucifera corolla sp. nov.(Alveolata, Perkinsozoa), a parasitoid of dinoflagellates. European Journal of Protistology.
• Paullada‐Salmerón, J. A., Cowan, M., Aliaga‐Guerrero, M., Gómez, A., Zanuy, S., Mañanos, E., & Muñoz‐Cueto, J. A. (2016). LPXRFa peptide system in the European sea bass: a molecular and immunohistochemical approach. Journal of Comparative Neurology, 524(1), 176-198.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.