HigherPurity™ Soil DNA Isolation Kit

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 100 Collection tubes (2 mL)
– 50 Dry bead tube
– 12 g Glass Bead
– 40 mL Lysis Solution 1 (LS1)
– 20 mL WB1 Buffer
– 35 mL EB Buffer
– 15 mL Inhibitor Removal Buffer (IR-Buffer)
– 50 microcentrifuge tube (1.5 mL)
– 15 mL Buffer A
– 25 mL Buffer

Purified DNA suitable for all common Molecular Biology applications, such as:

• Digestion with restriction enzymes.
• Automated sequencing.
• PCR template.
• Southern Blots.

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• Tested for isolation of DNA from soil sample.
• The quantity and quality of purified DNA attend to:
  • Ratio 260/ 280 (1.8-2).
  • Agarose gel electrophoresis.

• Precautions: Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water.

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

• Withey, Z., & Gweon, H. S. (2024). Longitudinal bacterial community dynamics and sodium hypochlorite intervention in a newly built university building. Science of The Total Environment, 175349.
• Withey, Z., Awan, A., Duguma, N., Fell, E., Martinez, N. J., Neary, E., … & Gweon, H. S. (2023). Mycobial community assemblages in sink drains across a university campus. Environmental DNA, 5(1), 212-224.
• Prasedya, E. S., Kurniawan, N. S. H., Ardiana, N., Ilhami, B. T. K., Mulyaningsih, T., Astuti, S. P., … & Widyastuti, S. (2022). Effects of Fermented Seaweed Fertilizer Treatment on Paddy Amino Acid Content and Rhizosphere Microbiome Community. Fermentation, 8(9), 420.
• Prasedya, E. S., Kurniawan, N. S. H., Kirana, I. A. P., Ardiana, N., Abidin, A. S., Ilhami, B. T. K., … & Nikmatullah, A. (2022). Seaweed Fertilizer Prepared by EM-Fermentation Increases Abundance of Beneficial Soil Microbiome in Paddy (Oryza sativa L.) during Vegetative Stage. Fermentation, 8(2), 46.
• Del Álamo, A. C., Pariente, M. I., Molina, R., & Martínez, F. (2022). Advanced bio-oxidation of fungal mixed cultures immobilized on rotating biological contactors for the removal of pharmaceutical micropollutants in a real hospital wastewater. Journal of Hazardous Materials, 425, 128002.
• Withey, Zoe, et al. “Characterization of communal sink drain communities of a university campus.” Environmental DNA (2021).
• Семенов, М. В. (2019). Метабаркодинг и метагеномика в почвенно-экологических исследованиях: успехи, проблемы и возможности. Журнал общей биологии, 80(6), 403-417.
• Kononenko, N., Baranova, E., Dilovarova, T., Akanov, E., & Fedoreyeva, L. (2020). Oxidative Damage to Various Root and Shoot Tissues of Durum and Soft Wheat Seedlings during Salinity. Agriculture, 10(3), 55.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.