Horse-Power™ Taq DNA Polymerase

Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

 Includes for 500 U:
– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl₂ (1.5 mL)
– 1.5 mL Buffer (10x)

• Routine amplifications.
• Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
• Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
• PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).

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• Functionally tested in PCR.
• Undetected bacterial DNA (by PCR).
• Undetectable nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.

• Shipped in: Gel pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

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• Arjona-López, J. M., Capote, N., & López-Herrera, C. J. (2019). Improved real-time PCR protocol for the accurate detection and quantification of Rosellinia necatrix in avocado orchards. Plant and Soil, 1-8.
• Fagín, E., Bravo, I., Garrido, J. L., Rodríguez, F., & Figueroa, R. I. Scrippsiella acuminata versus Scrippsiella ramonii: A Physiological Comparison. Cytometry Part A.
• Ortiz‐Bustos, C. M., López‐Bernal, A., Testi, L., & Molinero‐Ruiz, L. Environmental and irrigation conditions can mask the effect of Magnaporthiopsis maydis on growth and productivity of maize. Plant Pathology.
• SERRA MARTÍNEZ,. (2017). Detección de posibles fuentes de inoculo de Fusicladium eriobotryae en níspero.
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• Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 127(3), 323-340.
• Aguilar, L., Gallegos, Á., Arias, C. A., Ferrera, I., Sánchez, O., Rubio, R., … & Pérez, C. (2019). Microbial nitrate removal efficiency in groundwater polluted from agricultural activities with hybrid cork treatment wetlands. Science of the total environment, 653, 723-734.
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• Negreiros, A. M. P., Sales Júnior, R., León, M., Melo, N. J. D. A., Michereff, S. J., Ambrósio, M. M. D. Q., … & Armengol, J. Identification and pathogenicity of Macrophomina species collected from weeds in melon fields in Northeastern Brazil. Journal of Phytopathology.
• Rêgo, T. J. S., Elena, G., Correia, K. C., Tovar‐Pedraza, J. M., Câmara, M. P. S., Armengol, J., … & Berbegal, M. Genetic diversity and population structure of Lasiodiplodia theobromae from different hosts in northeastern Brazil and Mexico. Plant Pathology.
• Flores, B., & Javier, F. (2018). Diagnóstico de enfermedades causadas por hongos en plantas de palmera y tipo palmera de uso ornamental, en un vivero de la Comunidad Valenciana.
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• Mora-Sala, B., Cabral, A., León, M., Agustí-Brisach, C., Armengol, J., & Abad-Campos, P. Survey, Identification and Characterization of cylindrocarpon-like asexual morphs in Spanish forest nurseries. Plant Disease, (ja).
• Robledo-Mahón, T., Aranda, E., Pesciaroli, C., Rodríguez-Calvo, A., Silva-Castro, G. A., González-López, J., & Calvo, C. (2018). Effect of semi-permeable cover system on the bacterial diversity during sewage sludge composting. Journal of Environmental Management, 215, 57-67.
• Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 1-18.
• Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal, 325, 257-269.
• Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Identification and Characterization of TALE Homeobox Genes in the Endangered Fern Vandenboschia speciosa. Genes, 8(10), 275.
• Navarro-Domínguez, B., Ruiz-Ruano, F. J., Camacho, J. P. M., Cabrero, J., & López-León, M. D. (2017). Transcription of a B chromosome CAP-G pseudogene does not influence normal Condensin Complex genes in a grasshopper. Scientific reports, 7(1), 17650.
• Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
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• Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal.

• Anaya Calvo-Rubio, G. (2017). Desarrollo y validación de un sistema diagnóstico rápido para la detección de alteraciones cromosómicas que provocan infertilidad en caballos basado en herramientas moleculares.
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• León-Ortega, M., & González-Wangüemert, M. (2015). Characterization of 10 new tetranucleotide microsatellite markers for the European eagle owl, Bubo bubo: Useful tools for conservation strategies. Biochemical Systematics and Ecology, 63, 109-112.
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• ALMENDROS, V. M. T. (2016). Identificación de especies de Dactylonectria e Ilyonectria asociadas al pie negro de la vid en Castilla la Mancha.
• Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.
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• Alaniz, S., Armengol, J., León, M., García-Jiménez, J., & Abad-Campos, P. (2009). Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine. Mycological research, 113(1), 16-23.
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• García-Carneros, A. B., García-Ruiz, R., & Molinero-Ruiz, L. (2014). Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower. Helia, 37(61), 205-214.
• Ortiz-Bustos, C. M., Testi, L., García-Carneros, A. B., & Molinero-Ruiz, L. (2016). Geographic distribution and aggressiveness of Harpophora maydis in the Iberian peninsula, and thermal detection of maize late wilt. European Journal of Plant Pathology, 144(2), 383-397.
• Soto, E. C., Molina-Alcaide, E., Khelil, H., & Yáñez-Ruiz, D. R. (2013). Ruminal microbiota developing in different in vitro simulation systems inoculated with goats’ rumen liquor. Animal Feed Science and Technology, 185(1), 9-18.
• Rodríguez-Ruano, S. M., Martín-Vivaldi, M., Martín-Platero, A. M., López-López, J. P., Peralta-Sánchez, J. M., Ruiz-Rodríguez, M., … & Martínez-Bueno, M. (2015). The Hoopoe’s uropygial gland hosts a bacterial community influenced by the living conditions of the bird. PloS one, 10(10), e0139734.
• Armengol, J., Vicent, A., León, M., Berbegal, M., Abad‐Campos, P., & García‐Jiménez, J. (2010). Analysis of population structure of Rosellinia necatrix on Cyperus esculentus by mycelial compatibility and inter‐simple sequence repeats (ISSR). Plant pathology, 59(1), 179-185.
• Pérez‐Sierra, A., López‐García, C., León, M., García‐Jiménez, J., Abad‐Campos, P., & Jung, T. (2013). Previously unrecorded low‐temperature Phytophthora species associated with Quercus decline in a Mediterranean forest in eastern Spain. Forest Pathology, 43(4), 331-339.
• González‐Domínguez, E., León, M., Armengol, J., & Berbegal, M. (2015). A Nested Polymerase Chain Reaction Protocol for in planta Detection of Fusicladium eriobotryae, Causal Agent of Loquat Scab. Journal of Phytopathology, 163(5), 415-418.
• Tello, M. L., Gramaje, D., Gómez, A., Abad-Campos, P., & Armengol, J. (2010). Analysis of phenotypic and molecular diversity of Phaeomoniella chlamydospora isolates in Spain. Journal of Plant Pathology, 195-203.
• Salem, I. B., Correia, K. C., Boughalleb, N., Michereff, S. J., León, M., Abad-Campos, P., … & Armengol, J. (2013). Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. Plant disease, 97(6), 737-743.
• González, M., Pérez‐Sierra, A., Serrano, M. S., & Sánchez, M. E. (2016). Two Phytophthora species causing decline of wild olive (Olea europaea subsp. europaea var. sylvestris). Plant Pathology.
• Fernández-Bedmar, Z., & Alonso-Moraga, A. (2016). In vivo and in vitro evaluation for nutraceutical purposes of capsaicin, capsanthin, lutein and four pepper varieties. Food and Chemical Toxicology, 98, 89-99.
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• Agustí-Brisach, C., Cabral, A., González-Domínguez, E., Pérez-Sierra, A., León, M., Abad-Campos, P., … & Armengol, J. (2016). Characterization of Cylindrodendrum, Dactylonectria and Ilyonectria isolates associated with loquat decline in Spain, with description of Cylindrodendrum alicantinum sp. nov. European Journal of Plant Pathology, 145(1), 103-118.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.