First Strand cDNA Synthesis Kit

Name: First Strand cDNA Synthesis Kit
SKU: PR008
Price: 399.91 EUR
Availability: InStock

Efficient & Sensitive complete System for First strand cDNA Synthesis from RNA templates

First-Strand cDNA Synthesis Kit is an Efficient & Sensitive System for First strand cDNA Synthesis from RNA templates. It includes s all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA).

First-Strand cDNA Synthesis Kit features two optimized mixes, RT Enzyme Mix and RT Reaction Mix. RT Enzyme Mix combines Reverse Transcriptase and RNase Inhibitor, while RT Reaction Mix contains dNTPs, oligo(dT)18 primers, random hexamers, MgCl2 and an optimized buffer. The synthesized single-stranded cDNA is suitable for real-time quantitative PCR applications. The Kit has been formulated to provide high yields of full-length cDNA product and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg to 5 μg of total RNA.

100 rxn

Price: 399.91 €

SKU: PR008 Categories: End Point PCR, Reverse Transcription

Detailed information:

Advantages & Features

Convenient: the Kit includes all the components needed.
Highly Sensitive: full length cDNA starting at 10 pg – 5 μg of total RNA, due a novel RNase H treatment.
Full-length first strand cDNA up to 10kb.
Optimized to increase sensitivity in RT-qPCR and RT-PCR assays.
Optimal reaction temperature: 50 °C
Avoids RNA template degradation.

Specifications

Includes

Includes for 50 rxn:
– 50 μL Reverse Transcriptase (200U/µL)
– 500 μL cDNA Synthesis Buffer (5x)
– 50 μL dNTP Mix (10 mM)
– 50 μL Hexamer Primer (200ng/µL)
– 50 μL Oligo (dT)20(50µM)
– 25 μL RNase Inhibitor (50U/µL)
– 1 mL Nuclease-free Water
– 250 µL DTT (0.1 M)

Download documentation

Datasheet

MSDS

Applications

• First Strand cDNA Synthesis for RT-PCR and RT-qPCR.
• Construction of full length cDNA Libraries.
• RNA Analysis.

Tables & Figures

Quality Control

The performance of First-Strand cDNA Synthesis Kit is tested in an RT reaction using Human Total RNA. The sensitivity of the kit is verified by the detection of GAPDH transcript in 100 fg total RNA and the product generated is visualized on Agarose Gel.

Advice

Storage, Shipping & Guarantee

Shipment at Gel Pack.
Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to reduce freeze-thaw cycles as less as possible.

Citations

Todorova, M. N., Savova, M. S., Mihaylova, L. V., & Georgiev, M. I. (2023). Icariin Improves Stress Resistance and Extends Lifespan in Caenorhabditis elegans through hsf-1 and daf-2-Driven Hormesis. International Journal of Molecular Sciences, 25(1), 352.
Saleem, M. A., Mustafa, M. S., & Mustafa, M. (2024). Promoter Hypermethylation of the BRCA1 Gene as a Novel Biomarker for Prostate Cancer. Cureus, 16(8).
Amirova, K., Dimitrova, P., Koycheva, I., Balcheva-Sivenova, J., & Georgiev, M. I. Modulation of Nrf2 expression in human neutrophils by Ballota nigra extract.
Savova, M. S., Todorova, M. N., Apostolov, A. G., Yahubyan, G. T., & Georgiev, M. I. (2022). Betulinic acid counteracts the lipid accumulation in Caenorhabditis elegans by modulation of nhr-49 expression. Biomedicine & Pharmacotherapy, 156, 113862.
Koycheva, I. K., Vasileva, L. V., Amirova, K. M., Marchev, A. S., Balcheva-Sivenova, Z. P., & Georgiev, M. I. (2021). Biotechnologically produced Lavandula angustifolia Mill. extract rich in rosmarinic acid resolves psoriasis-related inflammation through Janus kinase/signal transducer and activator of transcription signaling. Frontiers in pharmacology, 940.
Mladenova, S. G., Savova, M. S., Marchev, A. S., Ferrante, C., Orlando, G., Wabitsch, M., & Georgiev, M. I. (2022). Anti-adipogenic activity of maackiain and ononin is mediated via inhibition of PPARγ in human adipocytes. Biomedicine & Pharmacotherapy, 149, 112908.
Mladenova, Saveta G., et al. “Anti-adipogenic Effect of Alchemilla monticola is Mediated via PI3K/AKT Signaling Inhibition in Human Adipocytes.“ Frontiers in Pharmacology: 2179.
Savova, Martina S., et al. “Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.” Biomedicine & Pharmacotherapy 141 (2021): 111934.
Lago-Docampo, M., Tenorio, J., Hernández-González, I., Pérez-Olivares, C., Escribano-Subías, P., Pousada, G., … & Valverde, D. (2020). Characterization of rare ABCC8 variants identified in Spanish pulmonary arterial hypertension patients. Scientific Reports, 10(1), 1-13.
Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.