One-Step RT PCR Kit
Highly Efficient cDNA Synthesis and PCR in a convenient Single Tube
One-Step RT PCR Kit is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube. The kit includes a PCR MasterMix supplied in a 2x concentration to perform standard PCR.
MasterMix contains all the reagents needed for running PCR reactions (except PCR primers and template). In addition, a separate RT Mix composed by a balanced mixture of both RTase and RNase Inhibitor is also provided.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA Polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA Polymerase exponentially amplifies the double-stranded DNA template.
Price: 275.74 €
Detailed information:
Advantages & Features
- Higher specificity, sensitivity, and yield.
- Efficient: thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields.
- Unique: Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs.
- Highly Sensitive: PCR enhancers allowing sensitive low background amplification.
Specifications
Includes
– 2 x 1.25 mL One-Step PCR Mix (2x)
– 2 x 125 μL RT Mix
–Â 2 mL Water (RNase-free)
Download documentation
Datasheet MSDSApplications
- One-step RT-PCR.
- Amplification of GC-rich and complex templates.
Tables & Figures
Quality Control
- One step RT-PCR using eukaryotic total RNA as a template.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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