Blunt End DNA Cloning kits

Discover the efficiency of our Blunt End DNA cloning kits for the most accurate results

pSpark® DNA Cloning Kits are based on a breakthrough technology to clone without toxic genes, from less than 1 ng/kb, with minimum background (less than 1%) getting an Unprecedented high Efficiency. It have been designed to clone PCR fragments generated with any proofreading DNA Polymerases or Blend DNA Polymerases,  and compatible with blue/white Screening. Expensive Phosphorylated Primers are not needed.

Look our Blunt End DNA cloning products without toxic genes and based in a novel technology

All pSpark® DNA Cloning Kits contain both T7 and SP6 RNA Polymerase promoters flanking the Multiple Cloning region (MCS) for in vitro transcription of cloned DNA using either T7 or SP6 RNA Polymerases. Also, all vectors have the origin of replication of the filamentous phage f1. Synthesis of single-stranded DNA requires phage encoded gene II, X and V and is initiated at f1 ori. Also, all pSpark® DNA Cloning Kits have binding sites for pUC/M13 Forward and Reverse Primers and thus cloned insert can be amplified or sequenced with those Primers.

In several  vectors the MCS has been properly inserted within the alpha-peptide coding region of the enzyme beta-galactosidase for insertional inactivation of the alpha-peptide by Recombinant Clones thus allowing positive clones to be directly identified by Blue/White screening on X-Gal plates.