PBS (pH 7.4)

Name: PBS (pH 7.4)
SKU: BR0003
Availability: InStock

High Quality and sterile solution for Research and Further Manufacturing applications

Phosphate-Buffered Saline (PBS) (pH 7.4) is a high quality, sterile and convenient water-based salt solution containing sodium phosphate, sodium chloride, potassium chloride and potassium phosphate.

PBS is widely used Cell Culture Manufacturing, and Upstream and Downstream processes, in Cell Biology to maintain the osmolarity, in Immunoassays (ELISA, immuno-histochemical), to maintain the protein pH, to dissolve proteins and peptides samples. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.

Do you need different packaging, size, label or a regular supply? Please, Contact us.

Price: 21.92 € – 1,298.23 €

size

1 L (1X)

1 L (10X)

1 L (20X)

5 L (1X)

20 L (10X)

20 L (1X)

100 L (1X)

SKU: BR0003 Category: Buffers & Reagents

Detailed information:

Advantages & Features

Reliable: manufactured in an ISO 13485:2016 and GMP-certified facility to guarantee lot-to-lot consistency.
Versatility Empowered: caters to a wide range of applications inside Manufacturing and Research.
High Purity: due additional steps to eliminate impurities, offering low levels of endotoxins, and microbial contaminants.
Sterile: Filtered through a 0.22micron filter.
Quality Guaranteed: CoA for each lot of products manufactured supplied upon request.
Supply Chain Guaranteed: due our strategic location, and deep expertise managing regulatory agencies and shipping globally.
Increase process Efficiency and Reduce Costs: improve efficiency and reduce QC testing with larger batch volumes, reduce cycle times with just-in-time solutions and capacity constraints.
Highly experienced Regulatory and Technical expertise: that eliminates raw material risks and batch-to-batch inconsistencies.

Specifications

Formula:

Component Name	CAS	MW
Sodium Phosphate Dibasic	7558-79-4	141.96
Potassium Phosphate Monobasic	7778-77-0	136.09
Potassium Chloride	7447-40-7	74.55
Sodium Chloride	7647-14-5	58.44
WFI Quality Water	7732-18-5	18.02

Standards Analytics (1x):

Test	Specificitation	Method reference
Appearance	Clear and Colourless liquid	Ph. Eur. 2.2.1; 2.2.2 and 2.9.20.
pH	7,2 – 7,6	Potentiometry Ph Eur 2.2.3
Osmolarity	280 – 310 mOsm/kg	Ph. Eur. 2.2.35.
Bioburden	<0.1 CFU/mL	Ph. Eur. 2.6.12
Endotoxin testing	< 1 EU/mL	Ph. Eur. 2.6.14

Includes

BR0003, BR0004 and BR0017:
– 1,000 mL PBS Solution (1X, 10X or 20X) in PET bottle.

BR0344:
– 5 L PBS Solution (1X) in 5L graduated square containers with moulded hand grip.

BR0347 and BR0348:
– 20 L PBS Solution (1X or 10X) in 20L graduated square containers with moulded hand grip.

BR0350:
– 100 L PBS Solution (1X) in single-use bag with plastic drum.

Download documentation

Datasheet

MSDS

Applications

Designed for a wide range of Further Manufacturing and Research applications:

- - Cell culture manufacturing.
  - Upstream and Downstream processes.
  - Dilute substances to working concentration.
  - Used to immobilize a substance, as a protein, in a solid surface.
  - Immuno‐histochemical, ELISA and Western blot assays.
  - Microbiological procedures.

Tables & Figures

Quality Control

Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:

- Batch-to-batch consistency.
- Facilities audited regularly Quality audits.
- Lot specific CoAs and QA review previously to release a product.
- Material traceability, documented procedures and documented employee training.
- Equipment maintenance and monitoring records.

Request a Certificate of Analysis (CoA)

Advice

Storage, Shipping & Guarantee

Shipped at: Ambient temperature.
Storage: Room temperature (15 – 25 ºC).
Shelf life: 24 months from date of manufacturing.

Citations

Onorini, D., Leonard, C. A., Phillips Campbell, R., Prähauser, B., Pesch, T., Schoborg, R. V., … & Borel, N. (2023). Neisseria gonorrhoeae Coinfection during Chlamydia muridarum Genital Latency Does Not Modulate Murine Vaginal Bacterial Shedding. Microbiology Spectrum, e04500-22.
Fernandes, L. M. (2022). Análise da expressão de genes biomarcadores de bem-estar por hibridização in situ de ARN em paralarvas de cultivo de Octopus vulgaris (Cuvier, 1797) (Doctoral dissertation).
Murillo, A., Cuenca, R., Serrano, E., Marga, G., Ahmed, A., Cervantes, S., … & Pastor, J. (2020). Leptospira Detection in Cats in Spain by Serology and Molecular Techniques. International Journal of Environmental Research and Public Health, 17(5), 1600.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO) and Further Manufacturing. The product was not tested for use in diagnostics, drug development, terapeutic use, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website or Contact us.