TBS with Tween™ 20 (pH 7.4) (20x)

Name: TBS with Tween™ 20 (pH 7.4) (20x)
SKU: BR041-L
Price: 165.45 EUR
Availability: InStock

High Quality Molecular Biology Grade Buffer for a Reliable & Convenient use in a wide range of Applications

Tris Buffered Saline with Tween™ 20 (TBS-T) is the most commonly used Wash Buffers or Blocking Buffer used for applications such as Western Blot and ELISA Assays.

TBS-T is an optimal formulation of pH stabilizers, salts and detergents designed to remove excess material, decreasing non-specific background staining. It is preferably used with alkaline phospatase or peroxidase-conjugated antibodies.

Product available under ISO13485 / cGMP or with custom formulation, packaging or application. Learn more >

1 L

Price: 165.45 €

SKU: BR041-L Categories: Ready-to-use Format, Buffers & Reagents

Detailed information:

Advantages & Features

Reliable: rigorous quality control standards to guarantee lot-to-lot consistency.
High Quality: free of DNAse, RNase or protease contamination.
Really fast and easy procedure: results in a few seconds with minimal handling steps.
Safe: non-toxic to cells.
Accurate: eliminates variables in your laboratory work flow.

Specifications

Chemicals: Analytical Grade.
Format: Liquid, 20x Concentrate.
Composition 1x: 0.15 M NaCl, 0.050 M Tril-HCH, 1 % Tween™ 20
Volume: 1000 mL
pH: 7.4 ±0.05 at 25 ºC

Includes

– 1,000 mL TBS with Tween™ 20 (pH 7.4) (20x)

Download documentation

Datasheet

MSDS

Applications

Washing nitrocellulose membrane in Western Blot and Microtiter plate wells in ELISA Assays.
Blocking Buffer for plate-based Assays.

Tables & Figures

Quality Control

Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Advice

Storage, Shipping & Guarantee

Shipped at: Ambient Temperature.
Storage: in Dry Place at Room Temperature.
Recommendations: The salts in 20x solution are at a very high concentration. It has been found that at temperatures below 10ºC, the salts fall out of solution. If this occurs during your shipment conditions, please stir the product to get the salts back into solution.

Citations

Clua‐Ferré, L., De Chiara, F., Rodríguez‐Comas, J., Comelles, J., Martinez, E., Godeau, A. L., … & Ramón‐Azcón, J. (2022). Collagen‐Tannic Acid Spheroids for β‐Cell Encapsulation Fabricated Using a 3D Bioprinter. Advanced Materials Technologies, 2101696.
Velasco-Mallorquí, F., Rodríguez-Comas, J., & Ramón-Azcón, J. (2021). Cellulose-based scaffolds enhance pseudoislets formation and functionality. Biofabrication, 13(3), 035044.
Velasco-Mallorquı, Ferran, et al. “New volumetric CNT-doped gelatin–cellulose scaffolds for skeletal muscle tissue engineering.”
Gómez-Domínguez, D., Epifano, C., de Miguel, F., Castaño, A. G., Vilaplana-Martí, B., Martín, A., … & Rodríguez-Milla, M. A. (2020). Consequences of Lmna Exon 4 Mutations in Myoblast Function.
Machuca, M. A. O., Garibay, X. G. F., Castaño, A. G., De Chiara, F., Albors, A. H., Trias, J. B., & Azcon, J. R. (2019). Muscle-on-a-chip with on-site multiplexed biosensing system for in situ-monitoring of secreted IL-6 and TNF-α. Lab on a Chip.
Hernández-Albors, A., Castaño, A. G., Fernández-Garibay, X., Ortega, M. A., Balaguer, J., & Ramón-Azcón, J. (2019). Microphysiological sensing platform for an in-situ detection of tissue-secreted cytokines. Biosensors and Bioelectronics: X, 100025.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.