LDH Cytotoxicity Assay Kit

Name: LDH Cytotoxicity Assay Kit
SKU: CA0020
Availability: InStock

For a Sensitive, Reproducible & Easy-to-use Colorimetric assay for Cytotoxicity Detection

Canvax™ LDH Cytotoxicity Assay Kit offers a Sensitive, Reproducible & Easy-to-use Colorimetric method for Cytotoxicity Detection.

Lactate dehydrogenase (LDH) is a ubiquitous enzyme present in cytosol of a wide variety of organisms. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. The measurement of this enzyme can be useful for estimating biomass, counting cells, and determining the health of cells and tissues. LDH is widely used for cytotoxicity testing of cultured cells as well as viability testing.

This assay kit uses the formazan dye INT as a colorimetric indicator of enzyme activity. LDH strips an electron from lactate which is transferred to NAD to yield NADH. The NADH subsequently reduces the INT which causes a color change from yellow to red. This colorimetric reaction can be measured at 490 nm.

Price: 204.71 € – 544.82 €

size

400 assays

2000 assays

SKU: CA0020 Categories: Cell Based Assays, Cytotoxicity Assays

Detailed information:

Advantages & Features

Ideal for High-Throughput Screening.
Colorimetric: measures LDH release by formation of colored product.
Fast and Easy-to-use: simple one-step procedure.
Powerful: stable LDH enzyme activity as a Cytotoxic Marker.
Safe: Non-radioactive.

Specifications

Includes

Includes for 400 assays:
– 4 x 5 mL LDH Assay Buffer
– 4 x 75 μL Dye solution
– 4 mL Lysis solution
– 20 mL Stop solution

Download documentation

Datasheet

MSDS

Applications

For rapid determination of Cytotoxicity based on lactate dehydrogenase released into cell culture medium.
Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.

Tables & Figures

Quality Control

Strict quality controls in every manufacturing step to guarantee the highest quality and reproducibility.

Advice

Storage, Shipping & Guarantee

Shipped in: Gel Pack.
Storage: LDH Assay Buffer (-20 °C) and Lysis Solution & Stop solution (+4°C).
Recommendations: Protect the product from light.

Citations

López Baltanás, R. (2023). Influencia del suplemento de magnesio sobre el estrés oxidativo, la Inflamación y la disfunción vascular asociada a la enfermedad renal crónica: estudios in vivo e in vitro.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.