ROS Detection Assay Kit (DCFDA / H2DCFDA)

Name: ROS Detection Assay Kit (DCFDA / H2DCFDA)
SKU: CA093
Price: 239.18 EUR
Availability: InStock

Highly Sensitive & Safe Assay for Reactive Oxygen Species Measurement

ROS Detection Assay Kit is a highly sensitive and safe assay to measure Reactive Oxygen Species. ROS include a number of reactive molecules and free radicals, derived from molecular oxygen, that damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O2•-), hydrogen peroxide (H2O2), hydroxyl radical (HO•) and singlet oxygen (1O2), all of which are more reactive than oxygen (O2) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation.

It uses the cell permeant reagent Dichlorodihydrofluorescein-diacetate (H2DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, the acetyl groups on H2DCFDA are cleaved by intracellular esterase to yield the non-fluorescent compound which is rapidly oxidized to highly fluorescent 2’,7’-Dichlorodihydrofluorescein by ROS. The fluorescence intensity is proportional to the ROS levels within the cell cytosol.

5 × 96 assays

Price: 239.18 €

SKU: CA093 Categories: Cell Based Assays, Stress Oxidative

Detailed information:

Advantages & Features

Based on unique fluorescent probe H₂DCFDA.
Fast & Easy Protocol.
Safe: non-Radioactive, no special handling needed.
High-Throughput.
Accurate & Reproducible results.

Specifications

Includes

– 5 mg Fluorescent Substrate (H2DCFDA)
– 50 mL 10X Assay Buffer
– 500 μL Positive control (H2O2, 8.8M)
– 1 mL DMSO

Download documentation

Datasheet

MSDS

Applications

• Measurement of intracellular levels of ROS.

Tables & Figures

Quality Control

Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Advice

Storage, Shipping & Guarantee

Shipment Gel Pack.
Storage: 4 °C upon receipt.

Citations

Martínez Cid, P. (2024). Innovación en modelos in vitro: Resistencia a la ferroptosis en células de cáncer de ovario epitelial.
López Calvo, I. (2023). Estudio del papel de la ferroptosis en la resistencia al tratamiento quimioterapéutico en Cáncer de Ovario.
Parn, K. W. (2023). Safety and efficacy of oral epigallocatechin gallate in attenuating hypertension in spontaneously hypertensive rats via modulation of the intrarenal renin-angiotensin system (Doctoral dissertation, UTAR).
Balart-García, P., Aristide, L., Bradford, T., Beasley-Hall, P., Polak, S., Ribera, I., … & Fernandez, R. (2022). Genomic exaptation and convergent evolution paved the way to independent subterranean colonization across beetle lineages.
Luparello, C., Branni, R., Abruscato, G., Lazzara, V., Drahos, L., Arizza, V., … & Vazzana, M. (2022). Cytotoxic capability and the associated proteomic profile of cell-free coelomic fluid extracts from the edible sea cucumber Holothuria tubulosa on HepG2 liver cancer cells. EXCLI Journal, 21, 722-743.
López-Grueso, M. J., Lagal, D. J., García-Jiménez, Á. F., Tarradas, R. M., Carmona-Hidalgo, B., Peinado, J., … & Padilla, C. A. (2020). Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells. Redox Biology, 101737.
Criel, H. (2019). INTRACELLULAR ACCUMULATION OF QUERCETIN AND ITS IMPACT ON CELLULAR STRESS: AN IN VITRO STUDY (Doctoral dissertation, Ghent University).
Luparello, C., Asaro, D. M. L., Cruciata, I., Hassell-Hart, S., Sansook, S., Spencer, J., & Caradonna, F. (2019). Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells. International Journal of Molecular Sciences, 20(4), 804.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.