BL21 (DE3) Competent Cells
Chemically Competent Escherichia coli Cells optimized for Protein Expression of T7 RNA Polymerase-based systems
BL21 (DE3) Competent Cells are Chemically Competent Escherichia coliĀ Cells used for Protein Expression of T7 RNA Polymerase-based Systems. These cells are resistant to the lytic bacteriophages T1 and T5. The BL21 (DE3) strain is a derivative of E. coli B. It is deficient in both lon and ompT proteases resulting in superior isolation of intact Recombinant Proteins. The host is a lysogen of DE3 and, therefore, carries a chromosomal copy of the T7 RNA polymerase gene that is controlled by the lacUV5 promoter. The strain utilizes the T7 RNA promoter to control protein expression. IPTG is used to induce Expression of the T7 RNA Polymerase.
Genotype: FĀÆ ompT gal dcm lon hsdSB(rB- mB-) Ī»(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Price: 214.47 €
Detailed information:
Advantages & Features
- High-level of Protein Expression.
- Transformation Efficiency: ā„ 106 cfu/Ī¼g.
- Deficient in proteases Ion and OmpT.
Specifications
Genotype:Ā FĀÆ ompT gal dcm lon hsdSB(rB- mB-) Ī»(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Includes
– 10 ĀµL BL21 (DE3) Competent Cells Control Plasmid (0.1 ng/ĀµL)
– 10 x 200Ī¼L BL21 (DE3) Competent Cells
– Dry Ice
Download documentation
Datasheet MSDSApplications
- Protein Expression using the T7 Expression System.
Tables & Figures
Quality Control
- BL21(DE3) Competent Cells consistently yield > 1.0 Ć 106 colony-forming units/Āµg competent cells control plasmid when transformed with non-saturating amounts of DNA (0.2ng/100 ĀµL cells).
Advice
Storage, Shipping & Guarantee
- ShipmentĀ with Dry Ice.
- Storage:Ā Upon receipt of the kit, immediately store the components at -80 Ā°C.Ā Storage at -20 Ā°C will result in a significant decrease in transformation efficiency.Ā Please note that Competent Cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations.
Citations
Safety Statements
This product is developed, designed and soldĀ exclusively for Research purposes andĀ in vitroĀ use only (RUO). The product was notĀ tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
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