6X BX/ Loading buffer
For visual Tracking of DNA Migration during Electrophoresis
6X BX/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Bromophenol blue and Xylene Cyanol FF as tracking dye. Bromophenol blue migrates fast in the agarose gel and corresponds to the migration of a 300 – 500 bp long DNA fragment in a 1% agarose gel. Xylene cyanol FF migrates comparatively slower and corresponds to the migration of a 4,000 – 5,000 bp long DNA fragment in a 1% agarose gel.
EDTA is included in the solution to protect the sample from nuclease degradation. Glycerol is added to provide high density to the solution. Due to its high density, the sample settles at the bottom of the well. It also helps DNA samples to be confined in the well without diffusing out from it.
Price: 31.00 €
Detailed information:
Advantages & Features
- High quality.
- Proven performance for DNA Ladders preparation.
- Optimized to protect samples from nuclease degradation, provide high density and for sample confinement in the well without diffusing out from it.
Specifications
- Concentration: 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% Glycerol, 10 mM Tris-HCl (pH 7.6) and 60 mM EDTA.
Includes
– 5 x 1 mL of selected Loading Buffer
Download documentation
Datasheet MSDSApplications
- Preparation of DNA Ladders, Markers and Samples for loading on agarose or polyacrylamide gels.
Tables & Figures
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Quality Control
- Exempt of nuclease activities (endo-, exo and ribonucleases) guaranteed by appropriate quality tests.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: 4 °C.
Citations
- Ghadamnan, Elmira. Characterization of Tick-Associated Microbiota in Spain Using a Nanopore-based Metabarcoding Approach: Insights into Potential Zoonotic Pathogens. 2023.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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