HigherPurity™ FFPE DNA Isolation Kit

Name: HigherPurity™ FFPE DNA Isolation Kit
SKU: AN0160
Availability: InStock

For Easy, Rapid & Highly Efficient Extraction and Purification of DNA From FFPE samples

HigherPurity™ Formalin Fixation and Paraffin Embedding (FFPE) DNA Isolation Kit is an easy, rapid and highly efficient method for long-term preservation of most archived pathological specimens. It extraction involves two different phases: Deparaffinization and DNA extraction.

HigherPurity™ advanced technology omits the use of flammable and malodorous xylene or d-limonene commonly used for deparaffinization, a proprietary buffer formulation Deparaffinization solution is used for the complete dissolution of the wax to release the tissue.

Price: 210.48 € – 823.25 €

size

50 rxn

250 rxn

SKU: AN0160 Categories: DNA & RNA Extraction, DNA Spin Column based Purification

Detailed information:

Advantages & Features

High quality: of DNA obtained.
Very easy and rapid procedure: it takes 173 minutes to obtain the paraffin removal with minimal handling.
Safe: avoids xylene and other toxic solvents.
High purity of sample: complete removal of contaminants and inhibitors for reliable downstream applications.
Concentrated: low elution volume between 15-30 μL.
High durability: optimized for long-term preservation of most archived pathological specimens.
Complete solution: includes two different phases Deparaffinization and DNA extraction.

Specifications

Includes

Includes for 50 rxn:
– 30 mL Deparaffinization solution
– 10 mL Tissue Lysis Buffer
– 15 mL Lysis/ Binding Buffer
– 2 x 30 mg Proteinase K
– 18 mL Desinhibition Buffer
– 10 mL Wash Buffer
– 10 mL Elution Buffer
– 50 units MicroSpin Columns
– 100 units Collection tubes

Download documentation

Datasheet

MSDS

Applications

Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples.
Isolation of DNA from fresh and archived FFPE samples.
Isolation of DNA from specimen of object slides.
Typical downstream application: PCR, qPCR, NGS, STR analysis.

Tables & Figures

Quality Control

DNA isolated from tissue slice Paraffin-embedded (10 µm) quantified by Spectrophotometer and analysed by PCR.

Advice

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

Citations

Sevilla, A., Morales, M. C., Ezkurra, P. A., Rasero, J., Velasco, V., Cancho-Galan, G., … & Asumendi, A. (2020). BRAF V600E mutational load as a prognosis biomarker in malignant melanoma. PLOS ONE, 15(3), e0230136.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.