HigherPurity™ Stool DNA Isolation Kit

Name: HigherPurity™ Stool DNA Isolation Kit
SKU: AN0130
Availability: InStock

For a Reliable, Easy & Convenient High Quality DNA Isolation from Fresh or Frozen Stool Samples

HigherPurity™ Stool DNA Isolation Kit provides a reliable, easy and convenient technique to isolate high quality DNA from fresh or frozen stool samples. The kit uses HigherPurity™ breakthrough technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate.

Fecal samples are rapidly and efficiently lysed by bead beating. The sample DNA is then bound to the surface of a Spin Filter membrane and washed and the bound DNA is then desorbed from the surface of the Spin Filter column. The inhibitors of the downstream PCR will be removed by utilizing the DNA binding column and the buffers system included in the kit.

Price: 151.28 € – 519.09 €

size

50 rxn

100 rxn

250 rxn

SKU: AN0130 Categories: DNA & RNA Extraction, DNA Spin Column based Purification

Detailed information:

Advantages & Features

High purity: eliminates humic acid, polysaccharides, phenol compounds and enzyme inhibitors from stool samples.
Great performance: expected yields are 15 ~35 µg.
Efficient: fecal samples are lysed by bead beating.
Time-saving protocol: rapid isolation of ready-to-use DNA within 54 minutes with minimal handling steps.
Safe and convenient: avoids phenol/chloroform extraction.
Sample Size: 50~200 mg of fresh or frozen stool sample.
High Binding capacity: up to 60 µg DNA per column.
Extremely easy procedure: to isolate high quality DNA from fresh or frozen stool samples.

Specifications

Includes

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 100 Collection tubes (2 mL)
– 50 Bead Tube (200 mg glass beads / tube)
– 50 Microcentrifuge tube (1.5 mL)
– 20 mL Lysis Solution 1 (LS1)
– 7 mL Buffer A
– 15 mL Inhibitor Removal Buffer (IR-Buffer)
– 20 mL Buffer B
– 20 mL WB1 Buffer
– 20 mL EB Buffer
– 15 mg Proteinase K

Download documentation

Datasheet

MSDS

Applications

Purified DNA suitable for all common Molecular Biology applications, such as:

Digestion with restriction enzymes.
Automated sequencing.
PCR template.
Southern Blots.

Tables & Figures

Quality Control

– Tested for isolation of DNA from stool sample.
– The quantity and quality of purified DNA is checked by:

Ratio 260/ 280 (1.8-2).
Agarose gel electrophoresis.
Digestion with restriction endonucleases.

Advice

Precautions: Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water.

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

Citations

Sleha, Radek, et al. “Strong Antimicrobial Effects of Xanthohumol and Beta-Acids from Hops against Clostridioides difficile Infection In Vivo.” Antibiotics 10.4 (2021): 392.
Метатранскриптомика, М. (2019). МЕТАГЕНОМИКА И МЕТАБАРКОДИНГ–ЧТО МОЖНО НАЗЫВАТЬ ЭТИМИ ТЕРМИНАМИ И ГДЕ ИХ ГРАНИЦЫ?. ЖУРНАЛ ОБЩЕЙ БИОЛОГИИ, 80(6), 403-417.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.