FastPANGEA™ High Fidelity DNA Polymerase MasterMix
For a Robust, Fast & Extreme Performance for all PCR applications
FastPANGEA™ High Fidelity DNA Polymerase is a second generation High-fidelity DNA Polymerase that offers extreme performance for all PCR applications. It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. The error rate of FastPANGEA™ DNA polymerase is at least 50-fold lower than a normal Taq DNA Polymerase.
It possesses the 5’→ 3´ DNA polymerase activity, 3´→ 5´ exonuclease activity and it generates PCR products with blunt ends. It is also suitable for amplification of long amplicons such as 10-15 kb of genomic DNA.
Price: 371.00 €
Detailed information:
Advantages & Features
- Second generation High-Fidelity DNA Polymerase.
- Extreme Fidelity: error rate is more than 50 times more accurate than normal Taq DNA Polymerase.
- Robust: maximal success with minimal optimisation.
- Very Fast: extension times are 15-30 seconds/kb.
- Increased product yield: with minimal amount of enzyme.
- Versatile: ideal for routine PCR, as well as long or difficult templates.
Specifications
Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.
Includes
-4 x 1.25 mL FastPANGEA™ High Fidelity DNA Polymerase (2x)
– 500 μL DMSO (100%)
– 100 μL MgCl2 (25 mM)
*500 x 20 µl reactions
*200 x 50 µl reactions
Download documentation
Datasheet MSDSApplications
- PCR-Cloning
- Primers extension.
- Long or difficult amplification.
- High-Throughput PCR.
Tables & Figures
Quality Control
- Functionally tested in PCR.
- Undetected bacterial DNA (by PCR).
Advice
- Note: Highly recommended for cloning into pSpark® DNA cloning vectors.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- CHAABENE, R. B. Role of associated rhoptry proteins in Toxoplasma gondii invasion.
- Nyonda, M. A., Kloehn, J., Sosnowski, P., Krishnan, A., Lentini, G., Maco, B., … & Soldati-Favre, D. (2022). Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii. Cell reports, 40(7), 111224.
- Lentini, Gaëlle, et al. “Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.” Nature Communications 12.1 (2021): 1-17.
- Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
- García-Maceira, T., García-Maceira, F. I., González-Reyes, J. A., & Paz-Rojas, E. (2020). Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC Biotechnology, 20(1), 41.
- Otero Bilbao, A. (2014). Characterization of two metallocarboxypeptidases of M14D subfamily from bacterial and human origin.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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