Horse-Power™ Taq DNA Polymerase, Master Mix
High-Quality Enzyme manufactured in facilities certified ISO13485 and ISO9001 in ready-to-use Master Mix format
Horse-Power™ Taq DNA Polymerase Master Mix (2x) is an optimized, easy– and ready-to-use Master Mix manufactured under the best quality standards, ensuring consistency from lot to lot which guarantees reproducibility. It contains all PCR reaction components: TruePure™ dNTPs, PCR buffer, Mg2+ and Taq DNA Polymerase. Only primers and template need to be added. The convenient Master Mix (2x) formulation saves time and eliminates the risk of contamination due to a reduced number of pipetting steps required.
Horse-Power™ Taq DNA Polymerase is a versatile and thermostable recombinant enzyme produced in an E. coli strain. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
More Info about the Manufacture of our IVD Enzymes. Learn more.
Price: 81.20 € – 899.00 €
Detailed information:
Advantages & Features
- Reliable: Consistency from lot to lot thanks to our stringent manufacturing and quality control processes.
- Highest purity: Highly pure enzyme (≥98%) with ultra-low residual DNA content, undesired secondary activities, and bioburden load.
- Highest quality: One quality grade for all your uses, from academic research to clinical applications in diagnostics.
- Customizable: Available in different concentrations, bulk, and Lyophilization-friendly format. Contact us for a customized Master Mix at [email protected]
- Highly efficient enzyme: High activity, specificity, thermostability, and great performance in PCR.
- Thermostable enzyme: Half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: Preferentially adenine, without template at 3´ends, leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: Biotinylated, fluorescently labeled, etc.
- Molecular Weight: 94 kDa.
Specifications
Includes
P0035: 250 rxn* Horse-Power™ Taq DNA Polymerase Master Mix (2x)
P0036: 10,000 rxn* Horse-Power™ Taq DNA Polymerase Master Mix (2x)
*Includes all necessary reagents except template and primer DNA.
Download documentation
Datasheet MSDSBrochureApplications
- Routine amplifications. PCR and qPCR.
- Colony screening (see Horse-Power™ Red DNA Polymerase).
- Amplifications up to 5 kb using plasmid, viral, or genomic DNA as template.
- Amplification of DNA mixtures.
- Incorporation of labeled nucleotides.
- PCR fragments amplification for TA or GC cloning.
Tables & Figures
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Quality Control
- Functionally tested in PCR.
- Ultra-low residual host DNA (by qPCR).
- Exempt of nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.
- Control of Bioburden.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- Angelova, G., Stefanova, P., Brazkova, M., & Krastanov, A. (2023). Molecular and morphological characterization of Xylaria karsticola (Ascomycota) isolated from the fruiting body of Macrolepiota procera (Basidiomycota) from Bulgaria. Plos one, 18(6), e0287679.
- Petrova, S., & Petkova, M. (2023). Plant Traits of Tilia tomentosa Moench, Fraxinus excelsior L., and Pinus nigra JF Arnold as a Proxy of Urbanization. Forests, 14(4), 800.
- Mwangi, Z., Naeku, G., Mureithi, M., Onyambu, F., & Bulimo, W. (2022). Mutation patterns of resistance genes for macrolides, aminoglycosides, and rifampicin in nontuberculous mycobacteria isolates from Kenya. F1000Research, 11(962), 962.
- Petkova, M., Gotcheva, V., Dimova, M., Bartkiene, E., Rocha, J. M., & Angelov, A. (2022). Screening of Lactiplantibacillus plantarum Strains from Sourdoughs for Biosuppression of Pseudomonas syringae pv. syringae and Botrytis cinerea in Table Grapes. Microorganisms, 10(11), 2094.
- Petkova, M., Spasova-Apostolova, V., Masheva, V., Atanasova, D., & Tahsin, N. (2021). Endophytic colonization of Solanaceае family plants by fungal entomopathogen Beauveria bassiana strain 339 to control Colorado potato beetle (Leptinotarsa decemlineata Say). Bulgarian Journal of Agricultural Science, 27, 1.
- Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
- Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal.
- Ruiz Carrascoso, G. (2016). Características microbiológicas y clínico-epidemiológicas de enterobacterias productoras de carbapenemasa oxa-48 en el contexto de un brote hospitalario (Doctoral dissertation, Universidad Complutense de Madrid).
Safety Statements
This product is Manufactured by Canvax Reagents SLU, in facilities certified ISO13485 and ISO9001 and according to cGMP following ICHQ7. For further processing in clinical or diagnostic applications, but not suitable for administration to humans or animals.
For more info, please check its Material Safety Data Sheet
MSDS
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