HotBegan™ Hot Start Red DNA Polymerase MasterMix

Name: HotBegan™ Hot Start Red DNA Polymerase MasterMix
SKU: P0320
Price: 105.84 EUR
Availability: InStock

For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg, with visual Tracking of DNA Migration

HotBegan™ Hot Start Red DNA Polymease Master Mix (2x) is an optimized ready-to-use solution containing HotBegan™ Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and Stabilizers. It is inactive at room temperature and only requires addition of template, primers, and water.

HotBegan™ Hot Start Taq DNA Polymerase is a Taq DNA Polymerase bound to a proprietary antibody that blocks Polymerase activity until denaturation step occurs. The heat labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and nonspecific products.

MasterMix also contains an Agarose Loading Buffer including a Red Dye for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well Agarose Gels. Like the Taq polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of enzyme activities are detectable.

5 x 1.25 mL (250 rxn)

Price: 105.84 €

SKU: P0320 Categories: End Point PCR, MasterMixes

Detailed information:

Advantages & Features

Accurate & Fast visual Tracking of DNA Migration: thanks to its composition that includes Red Dye Buffer.
High specificity: minimizes unspecific amplification.
Efficient: prevents or minimizes primer-dimer and nonspecific products.
Great sensitivity: amplifies from femptograms of DNA targets.
Inactive: at Room Temperature.
Optimized
Powerful: amplification of targets up to 5 kb.
Convenient: available in kits and MasterMix solution.

Specifications

Assay Conditions: 25mM Tris-HCl pH9.0 at 25°C, 50mM KCl, 2mM MgCl2, 0.1mg/mL gelatine, 200 µM of dATP, dGTP, dTTP, 100µM[α32-P]dCTP (0.05µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes

Includes for 250 rxn:
– 5 x 1.25 mL HotBegan™ Hot Start Red DNA Polymerase MasterMix (2x)*
– 1.5 mL MgCl2 Solution (50mM)**

* Includes HotBegan™ Hot Start Taq DNA Polymerase, Red Dye buffer (2x), 0.4 mM of each dNTP, 5 mM Mg2+ and 5% Glycerol.

**Separate tube 50 mM MgCl2 solution is provided for further optimisation. In some cases, we recommend to optimize Mg2 + concentration.

Download documentation

Datasheet

MSDS

Applications

PCR fragments amplification for TA or GC Cloning.
Design for High Throughput Applications.
Amplification from a limited DNA template or low copy number genes.

Tables & Figures

Quality Control

Functionally tested in PCR.
Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.

Advice

Storage, Shipping & Guarantee

Shipped in: Gel pack.
Storage: -20 °C.

Citations

León, Maela, et al. “Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain.“ Agronomy 10.8 (2020): 1062.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.