Proteinase K, Lyophilized powder, =30 units/mg
Lyophilized powder, ≥30 units/mg protein
Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. It is a serine protease that exhibits broad cleavage specificity. With a molecular weight 28.9 kDa, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids.
It is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).
Price: 47.14 € – 318.71 €
Detailed information:
Advantages & Features
- Highly active and stable: > 30 units/mg protein (hemoglobin, pH 7.5, 37 °C).
- High Quality: free of RNases, DNases and Exonucleases contamination.
- Purified by chromatography.
- Lyophilised format.
- Low cutting specificity.
Specifications
- Unit Definition: Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
- Host:Â Komagataella phaffii (Pichia pastori).
- Appearance: White lyophilized powder.
Includes
Includes for 30 mg:
– 30 mg Proteinase K (Lyophilized powder, =30 units/mg)
Download documentation
Datasheet MSDSApplications
- Isolation of genomic DNA from cultured cells and tissues.
- Removal of DNases and RNases during DNA and/or RNA purification.
- Determination of enzyme locations.
Tables & Figures
Quality Control
- Enzyme activity is assayed by digesting hemoglobin at a concentration of 16.7 mg/mL in a solution of 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2.
- Free of detectable RNase, DNase and exonuclease activities.
Advice
Storage, Shipping & Guarantee
- Shipment at Ambient Temperature.
- Storage: 4 °C or -20 °C.
Citations
- Chekli, Y., Peron-Cane, C., Dell’Arciprete, D., Allemand, J. F., Li, C., Ghigo, J. M., … & Beloin, C. (2020). Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Scientific reports, 10(1), 1-13.
- Merinas-Amo, R. (2021). Papel de aditivos alimentarios en la modulaciĂłn de procesos degenerativos: estudios in vivo e in vitro.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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