M-MLV Reverse Transcriptase

Name: M-MLV Reverse Transcriptase
SKU: P0073
Price: 95.37 EUR
Availability: InStock

Encoded Moloney Murine Leukemia Virus, purified from E.coli (200 U/µL)

M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.

Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT.

MW: 69 KDa.

GMP-grade reagent also available. Learn more.

10000 U (200 U/µL)

Price: 95.37 €

SKU: P0073 Categories: End Point PCR, Reverse Transcription

Detailed information:

Advantages & Features

Concentration: 200 U/µL.
Full Solution: includes Reaction Buffer 5x.
High Quality: free of DNAse, RNase, DNA or protease contamination.
Purity: >95% as judged by SDS-polyacrylamide gels with blue staining.
Final Product Size: 7 kb or less.

Specifications

Unit definition:
One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37 ° C, using poly(A) oligo dT as a template primer.

Includes

Includes:
– M-MLV Reverse Transcriptase (200 U/μL)
– Reaction Buffer (10x)

Download documentation

Datasheet

MSDS

Applications

RT PCR.
Synthesis of cDNA.
mRNA 5’-end Mapping by Primer Extension Analysis.
End-labeling of DNA.
Dideoxynucleotide Sequencing.

Tables & Figures

Quality Control

Exempt of nucleases (endo, exo and ribonucleases) activities.
Ultra-low DNA contamination
Low bioburden
Purity: >95% as judged by SDS-polyacrylamide gels with blue staining.

Advice

Storage, Shipping & Guarantee

Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).

Citations

Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Túnez-Fiñana, I., & Paz-Rojas, E. (2021). Amino Terminal Recognition by a CCR6 Chemokine Receptor Antibody Blocks CCL20 Signaling and IL-17 Expression via β-arrestin.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.