SP6 Polymerase (20,000 U/ml)
High Quality & Proven Performance in RNA In Vitro Transcription Reactions
SP6 RNA Polymerase is a recombinant protein from SP6 bacteriophage produced in E. coli. Highly specific for the SP6 promoter that makes it distinct from those of analogous enzymes. It catalyzes efficiently the synthesis of RNA from double-stranded DNA.
GMP-grade is also available. Learn more
Price: 64.05 €
Detailed information:
Advantages & Features
- Isolated form a recombinant source.
- Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
- Flexible Quality Systems that covers from RUO to cGMP.
- High-Purity: ≥ 95% determined by SDS.
Specifications
Protein information
Predicted MW: 98,55 kDa.
Purity: ≥ 95% by SDS-PAGE.
Storage Buffer: 50mM Tris-HCl buffer (pH 7.9 at 25°C), 100mM NaCl, 20 mM β-ME, 1mM EDTA, 0.1% (v/v) Triton® X-100, 50% (v/v) glycerol.
Biological activity: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5 nmol of CTP into acid-insoluble product in 60 minutes at 37 °C.
Gene Information
Official Symbol: SP6 RNAP.
Specie SP6: Bacteriophage.
GenBank accession number: AY288927.2
NCBI Protein RefSeq: P06221.
Protein Family: Phage and mitochondrial DNA-dependent RNA Polymerase.
Includes
– 2,000 U SP6 RNA Polymerase (20U/µL)
Download documentation
Datasheet MSDSApplications
- In vitro mRNA synthesis (DNA sequence to transcript must be cloned downstream SP6 promoter).
- Probes generation for its ability to incorporate 32P, 35S and 3H and fluorescent nucleotide analogs.
- RNAse protection assay.
- RNA amplification.
- Obtention of RNA targets for translation procedures.
Tables & Figures
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.
Advice
Storage, Shipping & Guarantee
- Shipped in:Â Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
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