Vaccinia Capping Enzyme
A convenient ready-to-use Enzymatic capping System based on the Vaccinia virus Capping Enzyme (VCE)
Vaccinia Capping Enzyme is a kit that provides the required components to catalyze the addition of 7- methylguanylate cap structures (Cap 0) to the 5′ end of RNA. Vaccinia Capping Enzyme (VCE) is a heterodimeric protein produced in Escherichia coli.
GMP-grade is also available. Learn more
Detailed information:
Advantages & Features
- Convenient solution that includes all component required for succesful Enzymatic capping System based on the Vaccinia virus Capping Enzyme (VCE).
- Isolated form a recombinant source.
- Extensivelly quality controlled: Endo/Exonucleases/RNAses free.
- Flexible Quality Systems that covers from RUO to cGMP.
Specifications
Unit definition: One unit of Vaccinia Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C.
Includes
– 400 U Vaccinia Capping Enzyme (10 U/µL)
– Reaction Buffer 10x (500 mM Tris-HCl pH=8,0; 10 mM MgCl2; 50 mM KCl; 10 mM DTT)
– GTP (10 mM)
– S-adenosylmethionine (SAM) 40 mM
Download documentation
Datasheet MSDSApplications
- Labeling 5´ end of mRNA.
- Capping mRNA prior to translation assays/in vitro translation.
Tables & Figures
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.
Advice
Avoid exposure to constant temperature changes.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
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