T7 Digoxigenin RNA Labeling Kit
For Easy, Scalable & High-Quality RNA Synthesis with Modified Nucleotides
T7 Digoxigenin RNA Labeling Kit is designed to generate randomly Digoxigenin-modified RNA probes by in vitrotranscription with Dioxigenin-11-UTP.
Digoxigenin (DIG) is a steroid hapten derived from plants of the genus Digitalis (D. lanata, and the purple foxglove D. purpurea). It is widely used for generating non-radioactive probes and is an alternative to biotinylation.
Like biotin, DIG-labeled probes support both chromogenic and chemiluminescence detection formats. RNA probes are synthesized by in vitro transcription with DIG-11-UTP. These nucleotides are linked via a spacer arm to DIG. The resulting DIG-labeled molecules then function as hybridization probes in much the same manner as any other type of probe.
Detailed information:
Advantages & Features
- High-Quality Kit: High enzymatic purity, specificity, and activity.
- Scaleable: Increase the reaction size to produce milligram amounts of RNA.
- Easy and time-saving protocol with minimal handling steps.
- Convenient: Rnase Inhibitor included.
Specifications
Includes
– 2x 40 μl T7 RNA Polymerase mix
– 200 μl T7 10X Reaction Buffer
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 25 μl DIG-11-UTP (10mM)
– 100 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
- in situ hybridization.
- Southern blots.
- Microarray hybridization.
- Chromosome mapping.
Tables & Figures
Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
- Avoid freeze/thaw cycles.
Storage, Shipping & Guarantee
- Shipped in:Â Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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