PickMutant™ DNA Shuffling Kit
For a Reliable, Robust & highly Efficient Random Mutagenesis by DNA Shuffling
PickMutant™ DNA-Shuffling Kit is based on the method Developed by Stemmer. Stemmer introduced DNA shuffling, the first homologous recombination method, in 1994.
DNA shuffling involves the digestion of a gene by DNase I into random fragments, and the reassembly of those fragments into a full-length gene by primerless PCR: the fragments prime on each other based on sequence homology, and recombination occurs when fragments from one copy of a gene anneal to fragments from another copy, causing a template switch, or crossover event. While randomly recombining the DNA sequences, the technique also introduces new point mutations at a relatively high rate (0.7%).
DNA shuffling consists of four steps: (i) preparation of genes to be shuffled, (ii) fragmentation with DNase I, (iii) reassembly by thermocycling in the presence of a DNA polymerase, and (iv) amplification of reassembled products by a conventional PCR.
Price: 320.02 €
Detailed information:
Advantages & Features
- Highly Efficient.
- Reliable.
- Robust.
Specifications
Includes
– 40 μL Taq Polymerase (5 U/μL)
– 500 μL PCR Buffer (10x)
– 80 μL dNTP Mix (10 mM)
– 100 μL DNase I Solution (0.1 U/ μL)
– 200 μL Reaction Buffer (10x)
– 100 μL Stop Solution
– 3 x 1.5 mL PCR-grade Water
Download documentation
Datasheet MSDSApplications
- Generation of a library of mutants.
- Slightly different genes coding for the same product.
- Homologous recombination of genes.
- Generation of gene libraries.
- Recombination of sequence blocks.
- In vitro Molecular evolution.
Tables & Figures
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Quality Control
- Tested using the insert control DNA provided.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
Citations
Wong, T. S., & Tee, K. L. (2020). Continuing from Protein Variants. In A Practical Guide to Protein Engineering (pp. 187-195). Springer, Cham.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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